Protein Info for Psest_0597 in Pseudomonas stutzeri RCH2

Annotation: RND family efflux transporter, MFP subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 483 signal peptide" amino acids 1 to 33 (33 residues), see Phobius details PF19335: HMBD" amino acids 44 to 70 (27 residues), 36.6 bits, see alignment (E = 9e-13) PF16576: HlyD_D23" amino acids 104 to 312 (209 residues), 230.8 bits, see alignment E=2.8e-72 PF16572: HlyD_D4" amino acids 150 to 202 (53 residues), 81.8 bits, see alignment 6.2e-27 TIGR01730: efflux transporter, RND family, MFP subunit" amino acids 208 to 386 (179 residues), 128.5 bits, see alignment E=1.4e-41 PF13437: HlyD_3" amino acids 208 to 309 (102 residues), 65.6 bits, see alignment E=1.5e-21 PF11604: CusF_Ec" amino acids 410 to 478 (69 residues), 74.3 bits, see alignment E=1.4e-24

Best Hits

KEGG orthology group: K07798, Cu(I)/Ag(I) efflux system membrane protein CusB (inferred from 61% identity to psa:PST_2082)

Predicted SEED Role

"Cobalt/zinc/cadmium efflux RND transporter, membrane fusion protein, CzcB family" in subsystem Cobalt-zinc-cadmium resistance

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GGR3 at UniProt or InterPro

Protein Sequence (483 amino acids)

>Psest_0597 RND family efflux transporter, MFP subunit (Pseudomonas stutzeri RCH2)
MTFQHKRLVLAFSLPLMISAAALIGLPTAAFGQSPAEEDKEVLYWYDPMVPQQKFDKPGK
SPFMDMELIPRYADEGSDGASISIDPSVTQNLGVRFATVTRESISQSLEATGALMFDERD
VAVVQARAGGFVERVYDRAPEDVIEKGAPLADLLVPEWVAAQEEYLALKGIGEPQLLAAA
RQRLRLAGMPPEVIVQLERTGKARPVWTVRSPIGGVLNSLDVREGMTVSTGASLARVNGL
EKVWLEVAVPEAQVANVAPGQLVNARLPAFAGEVLEGTIQAVLPQANLDSRTVRVRVELP
NPQQRLRPGMTAEVTLSRNVEDVLVIPSEAVIRTGRRALVMLAEDQGRYRPIEVRTGREF
DDQTEVLEGLEAGQKVVASGQFLLDSEASLRGLTAQGLEEPATSTEPALHEAEGTIVSLE
DGMVGLSHGPFKTLNMPGMTMSFPVADQSLLTKLQTGDHVRVGARESEEGLVIEHIEKLG
GQR