Protein Info for PGA1_c05990 in Phaeobacter inhibens DSM 17395

Annotation: formate--tetrahydrofolate ligase Fhs

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01268: FTHFS
  • amino acids 4 to 558 (555 residues), 903.8 bits, E=1.6e-276

Best Hits

Swiss-Prot: 93% identical to FTHS_RUEST: Formate--tetrahydrofolate ligase (fhs) from Ruegeria sp. (strain TM1040)

KEGG orthology group: K01938, formate--tetrahydrofolate ligase [EC: 6.3.4.3] (inferred from 93% identity to sit:TM1040_2358)

MetaCyc: 61% identical to formate--tetrahydrofolate ligase subunit (Clostridium cylindrosporum)
Formate--tetrahydrofolate ligase. [EC: 6.3.4.3]

Predicted SEED Role

"Formate--tetrahydrofolate ligase (EC 6.3.4.3)" in subsystem Folate Biosynthesis or One-carbon metabolism by tetrahydropterines or Serine-glyoxylate cycle (EC 6.3.4.3)

MetaCyc Pathways

• folate transformations III (E. coli) (9/9 steps found)
• folate transformations II (plants) (10/11 steps found)
• folate polyglutamylation (5/5 steps found)
• superpathway of tetrahydrofolate biosynthesis and salvage (10/12 steps found)
• formate assimilation into 5,10-methylenetetrahydrofolate (3/3 steps found)
• tetrahydrofolate salvage from 5,10-methenyltetrahydrofolate (2/2 steps found)
• formaldehyde oxidation VII (THF pathway) (3/4 steps found)
• L-histidine degradation III (4/6 steps found)
• folate transformations I (9/13 steps found)
• reductive glycine pathway of autotrophic CO₂ fixation (6/9 steps found)
• purine nucleobases degradation II (anaerobic) (15/24 steps found)
• reductive acetyl coenzyme A pathway I (homoacetogenic bacteria) (4/10 steps found)
• purine nucleobases degradation I (anaerobic) (5/15 steps found)
• Methanobacterium thermoautotrophicum biosynthetic metabolism (21/56 steps found)

KEGG Metabolic Maps

• Glyoxylate and dicarboxylate metabolism
• One carbon pool by folate

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.4.3

Sequence Analysis Tools

See I7DY17 at UniProt or InterPro

Protein Sequence (558 amino acids)

>PGA1_c05990 formate--tetrahydrofolate ligase Fhs (Phaeobacter inhibens DSM 17395)
MSYKSDIEIAREAQKRPIQEIGEKIGISSDDLLPYGHDKAKVSQEFINSVQSKEDGKLIL
VTAINPTPAGEGKTTTTVGLGDGLNRIGKNAMICIREASLGPNFGMKGGAAGGGYAQVVP
MEEMNLHFTGDFHAITSAHSLLSAMIDNHIYWGNECEIDIRRVAWRRVVDMNDRALRQIT
ASLGGVSNGFPRETGFDITVASEVMAILCLANDLKDLEKRLGDIIVAYRRDKTPVYCRDI
KAEGAMTVLLKDAMQPNLVQTLENNPAFVHGGPFANIAHGCNSVIATKTALKVADYVVTE
AGFGADLGAEKFMNIKCRKAGIAPSAVVLVATVRAMKMNGGVAKADLGAENVDAVNSGCA
NLGRHIENIKSFGVPVVVAINHFVTDTDAEVDAVKAYAATHGVEAVLSRHWELGSEGSAP
LAEKVVEIIEGGSANFAPIYPDDMPLFEKIETIAKRIYRADEVLADNKIRNQLKEWEEAG
YGNLPVCMAKTQYSFSTDPSLRGAPTGHSVPVREVRLSAGAGFIVAVCGEIMTMPGLPRK
PASESIRLNDDGQIEGLF