Protein Info for PGA1_c05870 in Phaeobacter inhibens DSM 17395

Annotation: holliday junction ATP-dependent DNA helicase RuvB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 349 PF05496: RuvB_N" amino acids 31 to 189 (159 residues), 261.7 bits, see alignment E=6.5e-82 TIGR00635: Holliday junction DNA helicase RuvB" amino acids 36 to 337 (302 residues), 438.9 bits, see alignment E=4.3e-136 PF07728: AAA_5" amino acids 66 to 183 (118 residues), 26.7 bits, see alignment E=1.6e-09 PF00004: AAA" amino acids 67 to 185 (119 residues), 80.1 bits, see alignment E=6.4e-26 PF17864: AAA_lid_4" amino acids 192 to 265 (74 residues), 96.4 bits, see alignment E=2e-31 PF05491: RuvB_C" amino acids 267 to 336 (70 residues), 89.1 bits, see alignment E=4.3e-29

Best Hits

Swiss-Prot: 95% identical to RUVB_RUEST: Holliday junction ATP-dependent DNA helicase RuvB (ruvB) from Ruegeria sp. (strain TM1040)

KEGG orthology group: K03551, holliday junction DNA helicase RuvB (inferred from 95% identity to sit:TM1040_2371)

MetaCyc: 62% identical to Holliday junction branch migration complex subunit RuvB (Escherichia coli K-12 substr. MG1655)
3.1.22.4-RXN [EC: 3.1.21.10]

Predicted SEED Role

"Holliday junction DNA helicase RuvB" in subsystem DNA-replication or RuvABC plus a hypothetical

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.1.21.10

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See I7EUE4 at UniProt or InterPro

Protein Sequence (349 amino acids)

>PGA1_c05870 holliday junction ATP-dependent DNA helicase RuvB (Phaeobacter inhibens DSM 17395)
MRESGTMIDADPTLRPTPLPEDSAAENDRALRPQGLGEFIGQAEARANLRVFIESARRRG
EAMDHTLFHGPPGLGKTTLAQIVARELGVNFRMTSGPVLAKAGDLAAILTNLESRDVLFI
DEIHRLNPAVEEVLYPAMEDFELDLVIGEGPAARTVRIELQPFTLVGATTRMGLLTTPLR
DRFGIPTRLQFYTVDELFEIVSRNARKLGAPADDDGAREIARRARGTPRIAGRLLRRVVD
FAVVEGDGTITRELADGALTRLGVDQLGLDGADRRYLRMVAENYGGGPVGIETMSAALSE
SRDALEEVIEPYLLQQGLIQRTPRGRMLAHKAWTHLGLTPPRGQGDLLL