Protein Info for Psest_0573 in Pseudomonas stutzeri RCH2

Annotation: cation diffusion facilitator family transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 296 signal peptide" amino acids 1 to 33 (33 residues), see Phobius details transmembrane" amino acids 42 to 62 (21 residues), see Phobius details amino acids 82 to 101 (20 residues), see Phobius details amino acids 114 to 136 (23 residues), see Phobius details amino acids 160 to 177 (18 residues), see Phobius details amino acids 183 to 200 (18 residues), see Phobius details TIGR01297: cation diffusion facilitator family transporter" amino acids 12 to 289 (278 residues), 229.2 bits, see alignment E=3.1e-72 PF01545: Cation_efflux" amino acids 17 to 208 (192 residues), 133.2 bits, see alignment E=1.1e-42 PF16916: ZT_dimer" amino acids 213 to 288 (76 residues), 86.8 bits, see alignment E=8.7e-29

Best Hits

Swiss-Prot: 50% identical to FIEF_CITK8: Cation-efflux pump FieF (fieF) from Citrobacter koseri (strain ATCC BAA-895 / CDC 4225-83 / SGSC4696)

KEGG orthology group: None (inferred from 95% identity to psa:PST_3715)

MetaCyc: 48% identical to Zn2+/Fe2+/Cd2+ exporter (Escherichia coli K-12 substr. MG1655)
RXN0-6; TRANS-RXN0-200; TRANS-RXN0-244

Predicted SEED Role

"Cobalt-zinc-cadmium resistance protein" in subsystem Cobalt-zinc-cadmium resistance

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GIN8 at UniProt or InterPro

Protein Sequence (296 amino acids)

>Psest_0573 cation diffusion facilitator family transporter (Pseudomonas stutzeri RCH2)
MSINRDHARLMRRATAAALATAIVLALSKAVAWWLSGSVSLLAGLTDSLLDGAASLLNLL
AVHYSLRPADDDHRYGHGKAEALAGLGQAAFICVSAILVGVQGVDRLLHPQPLGAQGVGI
AVMLFSLVMTAVLLSYQHHVVKVTGSTAIRADSLHYRSDLLLNTSILVALILAGFGWERL
DALFGIGIALYIFWSAITIVREAGAVLMDTELSPEISEQMQQLVCEVPGVHGCHDFRTRI
SGTRWFVQLHLELPGELPLSQAHDLCVAVENAIHDRYPNAEVLVHADPLEVAPPKR