Protein Info for PGA1_c05790 in Phaeobacter inhibens DSM 17395

Annotation: primosomal protein N

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 731 PF17764: PriA_3primeBD" amino acids 15 to 104 (90 residues), 48.7 bits, see alignment E=1.7e-16 PF04851: ResIII" amino acids 196 to 360 (165 residues), 39.1 bits, see alignment E=2.2e-13 PF00270: DEAD" amino acids 199 to 362 (164 residues), 65.3 bits, see alignment E=1.7e-21 TIGR00595: primosomal protein N'" amino acids 221 to 727 (507 residues), 597.1 bits, see alignment E=1.3e-183 PF18319: PriA_CRR" amino acids 447 to 473 (27 residues), 31.8 bits, see alignment (E = 3.2e-11) PF18074: PriA_C" amino acids 635 to 727 (93 residues), 60.5 bits, see alignment E=7.2e-20

Best Hits

KEGG orthology group: K04066, primosomal protein N' (replication factor Y) (superfamily II helicase) [EC: 3.6.4.-] (inferred from 83% identity to sit:TM1040_2378)

Predicted SEED Role

"Helicase PriA essential for oriC/DnaA-independent DNA replication" in subsystem Conserved gene cluster associated with Met-tRNA formyltransferase or DNA-replication

Isozymes

Compare fitness of predicted isozymes for: 3.6.4.-

Use Curated BLAST to search for 3.6.4.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See I7DY02 at UniProt or InterPro

Protein Sequence (731 amino acids)

>PGA1_c05790 primosomal protein N (Phaeobacter inhibens DSM 17395)
MQEQAFFNEGERVGVLTTQPLDRCLDYRAPEGGCHLGAYVEVPLGPRRVLGVIWGPGAGD
FDSAKLRPVNRVLDVAPMRDEMRQFLTKSADYTLTPLPAMLRLATRAPGLSDPVSMRKVL
RPTGQMPDRMTDARERVLSAVEEYGGLAFTAKELADLAGVTTSVVKGLVKQGALREEEAP
RDLPYPRLDPDLPGKTLTEDQAAAANTLGEGVAGGRYGTTLLKGVTGSGKTEVYLEAVAA
TLRAGRQALVLLPEIALTAEFLKRVEQRFGATPAEWHSGATMTERRRVWRMVGQGKAQLV
IGARSALFLPYQNLGLIIVDEEHDTSYKQDEGVCYNARDMAVLRAAMCSAQVVLASATPS
LETWANAEAGKYKRLDLAARFGSAVLPEMRAIDMRAEKLPASTWISPTLKQAMTARIERG
EQSLLFLNRRGFAPVTICRACGGHVTCDHCDSRMVEHRFMKRLMCHQCGETKPVPDACPT
CQVEGKMAPVGPGIERLGEETAALFPEARIAVLSSDLFASARALKQRIEEIAAGEADIIL
GTQLVAKGHNFPLLTLVGVIDADLGLQGSDLRAAERTFQLMRQVAGRAGRADKPGEALMQ
TFQPEHPVIRAILSGDEEAFWRAEAAERQAAGVPPYGRMAGIVLSGPELPAVMDLGMALA
RNDGPLRQIGAQVFGPAPAPIARIRGRHRVRLLIKASKGTPLQDAIARWIKPARLKGDLR
LSVDIDPQNFF