Protein Info for PGA1_c05710 in Phaeobacter inhibens DSM 17395

Annotation: isoleucyl-tRNA synthetase IleS

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 976 TIGR00392: isoleucine--tRNA ligase" amino acids 22 to 887 (866 residues), 774.2 bits, see alignment E=9.3e-237 PF00133: tRNA-synt_1" amino acids 35 to 694 (660 residues), 410.2 bits, see alignment E=2.7e-126 PF09334: tRNA-synt_1g" amino acids 72 to 219 (148 residues), 41.3 bits, see alignment E=2.6e-14 amino acids 626 to 686 (61 residues), 23 bits, see alignment 9.9e-09 PF08264: Anticodon_1" amino acids 738 to 887 (150 residues), 87.7 bits, see alignment E=2.2e-28 PF06827: zf-FPG_IleRS" amino acids 946 to 972 (27 residues), 23.5 bits, see alignment (E = 1.2e-08)

Best Hits

KEGG orthology group: K01870, isoleucyl-tRNA synthetase [EC: 6.1.1.5] (inferred from 77% identity to sit:TM1040_2389)

Predicted SEED Role

"Isoleucyl-tRNA synthetase (EC 6.1.1.5)" (EC 6.1.1.5)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See I7DMI2 at UniProt or InterPro

Protein Sequence (976 amino acids)

>PGA1_c05710 isoleucyl-tRNA synthetase IleS (Phaeobacter inhibens DSM 17395)
MCADTPQTPEYKDTLNLPKTDFPMRAGLPKREPGWLERWAQIGVYDKLREKAAAAKGTDA
ERKPFTLHDGPPYANGHLHIGHALNKTIKDMIVRSHQMMGFDARYVPGWDCHGLPIEWKI
EEQYRKKGKNKDEVPVVDFRQECRAFADEWVGIQREEFKRLGITGNWDDPYLTMNYHAEA
VIAGEFQKFLMNGTLYQGSKPVMWSPVEKTALAEAEIEYHDHKSHTIWVPFGTANATGDL
AKARVVIWTTTPWTIPSNKAVAYNPKVAYGLYRVDATQEESWTTPGELYLFADALAADAL
EKSRVTEFTKVRDVEASEFDGLILNHPFKGIEGANGFWDYDVPMIDGDHVTDDAGTGFVH
TAPSHGADDYECFVARNWIDRMTHNVGEESEFLPHVPFFAGLQVFDKKGKEGKANTAVIA
KLVEAGGIIARGRVTHSYPHSWRSKAPIVFRNTPQWFASVDRELDDGMGEMGDTIRTRAL
NSIDQLVQWTPQTGRNRLYSMIESRPDWVLSRQRAWGVPLTCFTKKGLLPTDPEFLLRNE
ALNTRIVAAFEEHGADVWYADGFKEKMLDGIANPEDYDQVFDVLDVWFDSGSTHAFVLRD
REDGTKDGIADVYMEGTDQHRGWFHSSLLQACGTKGRAPYRNVVTHGFTLDEKGNKMSKS
LGNTIVPEKIVQQYGADILRLWVAQTDYTADQRIGAEILKGVADGYRRLRNTMRYMLGNL
ADFSEDQRVDPADMPELEQWVLHRLAELDHKVRTGYQAYDFQGVFSAVFNFATVDLSAFY
FDVRKDALYCDGDTLNARAARTVLDILFHRLTTWLAPILVFTMEEVWLERFPGDDSSLHL
QDIPATPADWLNEPLAAKWAKVRQARRVVTAALEVQRVDKVIGASLEAAPVVHVRDAEVL
AALKSVNFADVCITSDIALSGDPAPAEAFRMPEVDGVSVVFEKADGDKCQRCWKILPDVG
NHSHAGVCGRCDAALS