Protein Info for Psest_0560 in Pseudomonas stutzeri RCH2

Updated annotation (from data): Urease (EC 3.5.1.5)
Rationale: Specifically important for utilizing Urea. Automated validation from mutant phenotype: the predicted function (3.5.1.5) was linked to the condition via a SEED subsystem. This annotation was also checked manually.
Original annotation: urease, gamma subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00547: Urease_gamma
  • amino acids 1 to 99 (99 residues), 156.9 bits, E=7.8e-51
• TIGR00193: urease, gamma subunit
  • amino acids 1 to 99 (99 residues), 172.1 bits, E=1.3e-55

Best Hits

Swiss-Prot: 97% identical to URE3_PSEU5: Urease subunit gamma (ureA) from Pseudomonas stutzeri (strain A1501)

KEGG orthology group: K01430, urease subunit gamma [EC: 3.5.1.5] (inferred from 91% identity to pae:PA4865)

MetaCyc: 68% identical to urease gamma subunit (Sinorhizobium meliloti Rm2011)
Urease. [EC: 3.5.1.5]

Predicted SEED Role

"Urease gamma subunit (EC 3.5.1.5)" in subsystem Urea decomposition (EC 3.5.1.5)

MetaCyc Pathways

• urea degradation II (1/1 steps found)
• superpathway of allantoin degradation in plants (4/8 steps found)

KEGG Metabolic Maps

• Arginine and proline metabolism
• Atrazine degradation
• Purine metabolism
• Urea cycle and metabolism of amino groups

Isozymes

Compare fitness of predicted isozymes for: 3.5.1.5

Use Curated BLAST to search for 3.5.1.5

Sequence Analysis Tools

See L0GHC4 at UniProt or InterPro

Protein Sequence (100 amino acids)

>Psest_0560 Urease (EC 3.5.1.5) (Pseudomonas stutzeri RCH2)
MDLSPREKDKLLIFTAGLVAERRLARGVRLNYPEAVAYISAALLEGARDGQTVADLMHYG
TTLLSREQVMEGVPEMIPEIQVEATFPDGTKLVTVHQPIA