Protein Info for Psest_0557 in Pseudomonas stutzeri RCH2

Updated annotation (from data): Urease (EC 3.5.1.5)
Rationale: Specifically important for utilizing Urea. Automated validation from mutant phenotype: the predicted function (3.5.1.5) was linked to the condition via a SEED subsystem. This annotation was also checked manually.
Original annotation: urease, alpha subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00449: Urease_alpha
  • amino acids 1 to 118 (118 residues), 189.7 bits, E=2.8e-60
• TIGR01792: urease, alpha subunit
  • amino acids 2 to 566 (565 residues), 996.8 bits, E=1.6e-304
• PF01979: Amidohydro_1
  • amino acids 124 to 450 (327 residues), 273 bits, E=6.5e-85

Best Hits

Swiss-Prot: 93% identical to URE1_PSEF5: Urease subunit alpha (ureC) from Pseudomonas fluorescens (strain ATCC BAA-477 / NRRL B-23932 / Pf-5)

KEGG orthology group: K01428, urease subunit alpha [EC: 3.5.1.5] (inferred from 99% identity to psa:PST_3729)

MetaCyc: 76% identical to urease alpha subunit (Sinorhizobium meliloti Rm2011)
Urease. [EC: 3.5.1.5]

Predicted SEED Role

"Urease alpha subunit (EC 3.5.1.5)" in subsystem Urea decomposition (EC 3.5.1.5)

MetaCyc Pathways

• urea degradation II (1/1 steps found)
• superpathway of allantoin degradation in plants (4/8 steps found)

KEGG Metabolic Maps

• Arginine and proline metabolism
• Atrazine degradation
• Purine metabolism
• Urea cycle and metabolism of amino groups

Isozymes

Compare fitness of predicted isozymes for: 3.5.1.5

Use Curated BLAST to search for 3.5.1.5

Sequence Analysis Tools

See L0GIM5 at UniProt or InterPro

Protein Sequence (566 amino acids)

>Psest_0557 Urease (EC 3.5.1.5) (Pseudomonas stutzeri RCH2)
MKISRQAYADMFGPTVGDKVRLADTDLWIEVEKDFTTYGEEVKFGGGKVIRDGMGQSQLC
AAEVVDTLITNALILDHWGIVKADVGLKDGRIAAIGKAGNPDIQPDVTIAIGASTEVIAG
EGMILTAGGIDSHIHFICPQQIEEALMSGVTTMIGGGTGPATGTNATTVTPGPWHMAMML
KAADAFPMNIGFTGKGNASLPEPLIEQVRAGAIGLKLHEDWGTTPAAIDNCLSVADQYDV
QVAIHTDTLNESGFVETTLGAFKGRTIHTYHTEGAGGGHAPDIIKACGFANVLPSSTNPT
RPFTRNTIDEHLDMLMVCHHLDPSIAEDVAFAESRIRRETIAAEDILHDLGAFSMISSDS
QAMGRVGEVITRTWQTADKMKKQRGALPGDGAGNDNFRAKRYIAKYTINPAITHGVSHEV
GSIEVGKWADLVLWRPAFFGVKPTLILKGGAIAASLMGDANASIPTPQPVHYRPMFASFG
GSLHASSFTFISQAAFEAGVPEQLGLKKKIGVVKGCRSVQKKDLIHNDYTPDIQVDPQNY
QVRADGQLLWCEPAEVLPMAQRYFLF