Protein Info for PGA1_c05620 in Phaeobacter inhibens DSM 17395

Annotation: putative 7-cyano-7-deazaguanosine biosynthesis protein QueE

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 265 TIGR03365: 7-cyano-7-deazaguanosine (preQ0) biosynthesis protein QueE" amino acids 32 to 265 (234 residues), 366.5 bits, see alignment E=3e-114

Best Hits

Swiss-Prot: 66% identical to QUEE_AGRFC: 7-carboxy-7-deazaguanine synthase (queE) from Agrobacterium fabrum (strain C58 / ATCC 33970)

KEGG orthology group: K10026, queuosine biosynthesis protein QueE (inferred from 75% identity to sit:TM1040_2045)

Predicted SEED Role

"Queuosine Biosynthesis QueE Radical SAM" in subsystem Queuosine-Archaeosine Biosynthesis

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See I7EUC4 at UniProt or InterPro

Protein Sequence (265 amino acids)

>PGA1_c05620 putative 7-cyano-7-deazaguanosine biosynthesis protein QueE (Phaeobacter inhibens DSM 17395)
MTEVLIPRAVRAAGDTSSPVNRQGASRSAPTTLRIAEIFGPTIQGEGALIGEPTVFVRAG
GCDYRCSWCDSLHAVDSRYRDTWVPMATDEVWQEVTRLSGGQPLTVSLSGGNPAIQNFAP
LIAQGKEAGYRFACETQGSISQPWFAQLDTLVLSPKPPSSGHKVDWQKFDHCLQAAKGCG
QTVVKIVIFDDRDYDWARVVAKRLPDLPLYLQPGNPEVDPDKPVDLTAATERLHWLIEKV
TADRWFTPRVLPQLHVLVWGNKRGV