Protein Info for Psest_0544 in Pseudomonas stutzeri RCH2

Annotation: Hydrogenase/urease accessory protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 190 signal peptide" amino acids 1 to 22 (22 residues), see Phobius details transmembrane" amino acids 33 to 55 (23 residues), see Phobius details amino acids 63 to 82 (20 residues), see Phobius details amino acids 88 to 107 (20 residues), see Phobius details amino acids 112 to 129 (18 residues), see Phobius details amino acids 141 to 162 (22 residues), see Phobius details amino acids 169 to 189 (21 residues), see Phobius details PF04955: HupE_UreJ" amino acids 11 to 187 (177 residues), 208.8 bits, see alignment E=2.4e-66

Best Hits

Swiss-Prot: 48% identical to HUPE_RHILV: Protein HupE (hupE) from Rhizobium leguminosarum bv. viciae

KEGG orthology group: K03192, urease accessory protein (inferred from 89% identity to psa:PST_3739)

Predicted SEED Role

"HupE-UreJ family metal transporter" in subsystem Transport of Nickel and Cobalt or Urea decomposition

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GGL9 at UniProt or InterPro

Protein Sequence (190 amino acids)

>Psest_0544 Hydrogenase/urease accessory protein (Pseudomonas stutzeri RCH2)
MNVQKILVTSALLLSPALALAHPGHDHAGVLSGIAHPIFGLDHLLAMLAVGLWAAQQTGK
ARWALPMTFVATMLLGGLAGFAGIEMPLMETGIAGSVLALGLLVALAVRPPLVVAAALTA
LFAASHGVAHGLELPELSSPWGYAAGFIVATAALHAAGYALVRYLPQAAAPLVRIAGAAS
ALTGAWLLAS