Protein Info for Psest_0541 in Pseudomonas stutzeri RCH2

Annotation: succinate CoA transferases

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 53% identical to CAT1_CLOK5: Succinyl-CoA:coenzyme A transferase (cat1) from Clostridium kluyveri (strain ATCC 8527 / DSM 555 / NCIMB 10680)

KEGG orthology group: None (inferred from 63% identity to gth:Geoth_3395)

MetaCyc: 53% identical to succinyl-CoA:CoA transferase (Clostridium kluyveri)
RXN-8807 [EC: 2.8.3.18]

Predicted SEED Role

"acetyl-CoA hydrolase/transferase family protein"

MetaCyc Pathways

TCA cycle VII (acetate-producers) (8/9 steps found)
pyruvate fermentation to acetate V (3/3 steps found)
acetate formation from acetyl-CoA (succinate) (1/1 steps found)
pyruvate fermentation to acetate VI (2/3 steps found)
anaerobic energy metabolism (invertebrates, mitochondrial) (5/10 steps found)
succinate fermentation to butanoate (2/7 steps found)
superpathway of anaerobic energy metabolism (invertebrates) (9/17 steps found)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.8.3.18

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GEJ0 at UniProt or InterPro

Protein Sequence (503 amino acids)

>Psest_0541 succinate CoA transferases (Pseudomonas stutzeri RCH2)
MATDLNKYIRNAAFLDKVVSAEEAASWIEDGMTLGMSGFTLFGEPKVFPKALSERGQREK
FKVNLFTGASMGPAADQSMAEAGIINKRIPYQGNAVQRKKINAGEVLYIDQHLSHTAELL
RQGVLPQVDYAIIEAAAITEDGQIIPTGSVGNSPIFVQNAKHVIIELNTSAPREYEGMHD
IYIPGPAGEDSRKDIPVYNVSKRIGSIGIPVDPAKVRGIVLSSEPDIPSPLFEPNAETQK
IADNLLDFLREEVKLGRLTNSLAPLQSGVGSVANAVLAGMKTSEFKDLTVASEVLQDGVF
DLIDAGVVKFAAATAFSLSKKRVDNLASDLAKYAGKVVFRPQEISNHPEVIRRLGIISFN
TALEADIYGNVNSTHVNGTHMMNGIGGSGDFARNARISIFVTTSTAKDGKISSIVPFVTH
VDHTNHDVDVIITEQGYADLRGLAPVEAAARVINNCMHPDYRAQALAYLEEAKQRGGHTP
HVLEKAFSWHANFAKNGTMLLDK