Protein Info for Psest_0532 in Pseudomonas stutzeri RCH2

Annotation: tRNA-N(6)-(isopentenyl)adenosine-37 thiotransferase enzyme MiaB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 437 TIGR01574: tRNA-i(6)A37 thiotransferase enzyme MiaB" amino acids 4 to 435 (432 residues), 591.6 bits, see alignment E=1.1e-181 TIGR00089: radical SAM methylthiotransferase, MiaB/RimO family" amino acids 4 to 433 (430 residues), 491.6 bits, see alignment E=1.9e-151 PF00919: UPF0004" amino acids 4 to 104 (101 residues), 99.3 bits, see alignment E=1.6e-32 PF04055: Radical_SAM" amino acids 151 to 320 (170 residues), 98.1 bits, see alignment E=9.7e-32 PF01938: TRAM" amino acids 373 to 436 (64 residues), 66.3 bits, see alignment E=2.7e-22

Best Hits

Swiss-Prot: 98% identical to MIAB_PSEU5: tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase (miaB) from Pseudomonas stutzeri (strain A1501)

KEGG orthology group: K06168, bifunctional enzyme involved in thiolation and methylation of tRNA (inferred from 98% identity to psa:PST_3761)

MetaCyc: 68% identical to isopentenyl-adenosine A37 tRNA methylthiolase (Escherichia coli K-12 substr. MG1655)
RXN0-5063 [EC: 2.8.4.3]

Predicted SEED Role

"tRNA-i(6)A37 methylthiotransferase" in subsystem Ribosomal protein S12p Asp methylthiotransferase or tRNA processing

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.8.4.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GIK0 at UniProt or InterPro

Protein Sequence (437 amino acids)

>Psest_0532 tRNA-N(6)-(isopentenyl)adenosine-37 thiotransferase enzyme MiaB (Pseudomonas stutzeri RCH2)
MTRKLFIETHGCQMNEYDSSRMVDLLGEHQALEITENPAEADVILLNTCSIREKAQEKVF
SQLGRWRELKLDNPQLVIGVGGCVASQEGAAIRDRAPYVDVVFGPQTLHRLPEMIDAART
TKKPQVDISFPEIEKFDRLPEPRVDGPSAFVSVMEGCSKYCTFCVVPYTRGEEVSRPLAD
VLAEIVHLAENGVKEVTLLGQNVNGYRHDGHDFADLLHAVAAIDGIGRIRYTTSHPLEFS
DAIIQAHADIPQLVKYLHLPVQAGSDRILAAMKRNHTALEYKSRIRRLRAAVPDILISSD
FIVGFPGETDKDFEQTMKLIEDVGFDFSYSFVYSARPGTPAADLIDDTPDEVKKQRLAIL
QQRINQQGFENSRRMVGTTQRILVSDYSKKDPGMLQGRTEHNRIVNFRCDNPRLIGQFVE
VHIDDALPHSLRGTLLS