Protein Info for HP15_511 in Marinobacter adhaerens HP15

Annotation: bifunctional riboflavin kinase/FMN adenylyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF06574: FAD_syn
  • amino acids 22 to 175 (154 residues), 176.2 bits, E=6.8e-56
• TIGR00083: riboflavin biosynthesis protein RibF
  • amino acids 28 to 313 (286 residues), 305.7 bits, E=3.6e-95
• PF01687: Flavokinase
  • amino acids 192 to 312 (121 residues), 139.1 bits, E=1.4e-44

Best Hits

Swiss-Prot: 55% identical to RIBF_PSEFL: Bifunctional riboflavin kinase/FMN adenylyltransferase (ribF) from Pseudomonas fluorescens

KEGG orthology group: K11753, riboflavin kinase / FMN adenylyltransferase [EC: 2.7.1.26 2.7.7.2] (inferred from 82% identity to maq:Maqu_0861)

MetaCyc: 51% identical to bifunctional riboflavin kinase / FMN adenylyltransferase (Escherichia coli K-12 substr. MG1655)
FAD synthetase. [EC: 2.7.7.2]; Riboflavin kinase. [EC: 2.7.7.2, 2.7.1.26]

Predicted SEED Role

"Riboflavin kinase (EC 2.7.1.26) / FMN adenylyltransferase (EC 2.7.7.2)" in subsystem Riboflavin, FMN and FAD metabolism (EC 2.7.1.26, EC 2.7.7.2)

MetaCyc Pathways

• flavin biosynthesis I (bacteria and plants) (8/9 steps found)
• flavin salvage (2/2 steps found)
• flavin biosynthesis III (fungi) (7/9 steps found)
• 5,6-dimethylbenzimidazole biosynthesis I (aerobic) (1/3 steps found)
• flavin biosynthesis II (archaea) (5/10 steps found)
• roseoflavin biosynthesis (1/7 steps found)
• adenosylcobalamin biosynthesis II (aerobic) (13/33 steps found)

KEGG Metabolic Maps

• Riboflavin metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.1.26 or 2.7.7.2

Sequence Analysis Tools

See E4PNF7 at UniProt or InterPro

Protein Sequence (323 amino acids)

>HP15_511 bifunctional riboflavin kinase/FMN adenylyltransferase (Marinobacter adhaerens HP15)
MRLIRGLTNLKMLSRREDSPLANGCVATIGNFDGVHLGHKTIIDQVKSKAKALGVPSVVM
IFEPQPREFFQGREAPPRLMGFRQKFEALLAEGIDIVLCLRFNQAFRSYSAMGFIDDVLI
HGLAVRHLVVGDDFRFGCDRAGDFALLEKVGYDAGFSVENTLTVTVDGERVSSTRVRDLL
TVNGLEKAESLLGHPYRIRGRVVYGRQLGRQIGAPTANILLHRTPALRGVYVVAATLDDG
ARFDGVANIGVRPTVDGKRPSLEVHLFDFAGTLYGQHLEVVFRHGLRDEEKFGSVDELKD
QIARDFDHARAWIAENGSVKSAD