Protein Info for Psest_0519 in Pseudomonas stutzeri RCH2

Annotation: lipoate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 325 TIGR00510: lipoyl synthase" amino acids 36 to 320 (285 residues), 434.2 bits, see alignment E=1.3e-134 PF16881: LIAS_N" amino acids 38 to 77 (40 residues), 33.8 bits, see alignment 4e-12 PF04055: Radical_SAM" amino acids 92 to 255 (164 residues), 86.3 bits, see alignment E=2.8e-28

Best Hits

Swiss-Prot: 99% identical to LIPA_PSEU5: Lipoyl synthase (lipA) from Pseudomonas stutzeri (strain A1501)

KEGG orthology group: K03644, lipoic acid synthetase [EC: 2.8.1.8] (inferred from 99% identity to psa:PST_3774)

MetaCyc: 65% identical to lipoyl synthase (Escherichia coli K-12 substr. MG1655)
Lipoyl synthase. [EC: 2.8.1.8]

Predicted SEED Role

"Lipoate synthase" in subsystem Lipoic acid metabolism

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.8.1.8

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GGJ3 at UniProt or InterPro

Protein Sequence (325 amino acids)

>Psest_0519 lipoate synthase (Pseudomonas stutzeri RCH2)
MSSVETAGKRPAKVEAGVKLRGAEKVARIPVKIIPTDELPKKPDWIRVRIPVSPEVDQIK
QTLRKHKLHSVCEEASCPNLGECFSSGTATFMIMGDICTRRCPFCDVGHGRPNALDPDEP
KNLAQAIADMRLKYVVITSVDRDDLRDGGAQHFADCLREIRKLSPSIQLETLVPDYRGRM
DIALDITASEPPDVFNHNLETVPRLYKSSRPGSDFEWSLDLLEKFKQRVPGVPTKSGLML
GLGETDEEVIEVMHRMREHDIDMLTLGQYLQPSRNHLPVQRFVHPDTFAWFAEEGMKMGF
KNIASGPLVRSSYHADQQAHGAKHD