Protein Info for HP15_487 in Marinobacter adhaerens HP15

Annotation: DNA repair protein RadA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 458 TIGR00416: DNA repair protein RadA" amino acids 1 to 452 (452 residues), 681.8 bits, see alignment E=2.1e-209 PF18073: Rubredoxin_2" amino acids 8 to 35 (28 residues), 39.8 bits, see alignment (E = 7.2e-14) PF06745: ATPase" amino acids 76 to 150 (75 residues), 40.6 bits, see alignment E=5e-14 PF13481: AAA_25" amino acids 85 to 223 (139 residues), 54.4 bits, see alignment E=3.4e-18 PF13541: ChlI" amino acids 346 to 431 (86 residues), 36.4 bits, see alignment E=1.1e-12 PF05362: Lon_C" amino acids 353 to 434 (82 residues), 31 bits, see alignment E=4.9e-11

Best Hits

Swiss-Prot: 71% identical to RADA_SALTY: DNA repair protein RadA (radA) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K04485, DNA repair protein RadA/Sms (inferred from 92% identity to maq:Maqu_0839)

Predicted SEED Role

"DNA repair protein RadA" in subsystem DNA repair, bacterial

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See E4PND3 at UniProt or InterPro

Protein Sequence (458 amino acids)

>HP15_487 DNA repair protein RadA (Marinobacter adhaerens HP15)
MAKTRTAYVCTECGADYSKWQGQCTACQAWNTISEVRGVSSNSKGARGGRFEGFAGSLSE
VQSLDDVSLAEQPRISSGMQEFDRVLGGGLVEGSAVLMGGHPGAGKSTLLLQAICHLAAS
VPALYVTGEESLQQVAMRAKRLGLPTKDLKMLSETSVERVMQVAEAEKPRILVVDSIQVM
HMADIESAPGSVSQVRESAAYLTRFAKQTGTILFLVGHVTKDGSLAGPKVLEHMIDCSIL
LEGSSDSRYRTLRGIKNRFGAVNELGVFAMLEQGLKEVKNPSAIFLNRGEDAAPGSVVMV
VWEGTRPMLVEIQALVDMAQGGYPRRVAVGLDQNRLAMLLAVLHRHGGMHVSDQDVFVNV
VGGVKVNETSADLALLAAIVSSFRDRALPQDLVIFGEVGLSGEIRPVPSGQERIYEAAKH
GFTRALVPKSNAPRKTIVGMKVIPVIKLSDALTALEEL