Protein Info for Psest_0483 in Pseudomonas stutzeri RCH2

Annotation: DNA topoisomerase IV, B subunit, proteobacterial

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 659 signal peptide" amino acids 1 to 21 (21 residues), see Phobius details TIGR01055: DNA topoisomerase IV, B subunit" amino acids 33 to 653 (621 residues), 1029.9 bits, see alignment E=0 PF02518: HATPase_c" amino acids 57 to 175 (119 residues), 54.2 bits, see alignment E=3.8e-18 PF00204: DNA_gyraseB" amino acids 247 to 414 (168 residues), 137 bits, see alignment E=9.9e-44 PF01751: Toprim" amino acids 442 to 551 (110 residues), 49.5 bits, see alignment E=7.8e-17 PF00986: DNA_gyraseB_C" amino acids 587 to 649 (63 residues), 82.6 bits, see alignment E=3.5e-27

Best Hits

Swiss-Prot: 92% identical to PARE_PSEAE: DNA topoisomerase 4 subunit B (parE) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K02622, topoisomerase IV subunit B [EC: 5.99.1.-] (inferred from 96% identity to psa:PST_3810)

Predicted SEED Role

"Topoisomerase IV subunit B (EC 5.99.1.-)" in subsystem DNA topoisomerases, Type II, ATP-dependent or Resistance to fluoroquinolones (EC 5.99.1.-)

Isozymes

Compare fitness of predicted isozymes for: 5.99.1.-

Use Curated BLAST to search for 5.99.1.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GI60 at UniProt or InterPro

Protein Sequence (659 amino acids)

>Psest_0483 DNA topoisomerase IV, B subunit, proteobacterial (Pseudomonas stutzeri RCH2)
MRPRRFIPVGVLFFSQSARANCSEHKDSARQMSYTAEAIEVLSGLDPVRKRPGMYTDTSR
PNHLAQEVIDNSVDEALAGHARSVQVILHQDNSLEVIDDGRGMPVDIHPEEGVPGVELIL
TKLHAGGKFSNKNYQFSGGLHGVGISVVNALSTRVEVTVKRDGSEYRMSFADGFKATDLE
VIGSVGKRNTGTSVRFWADPKYFDSPKFSISRLKHVLKAKAVLCPGLSVSFEDKGSGEKV
EWHYEDGLRSYLVDSVSDYLRLPDEPFVGTLAGNTEAVDWALLWLPEGGESVQESYVNLI
PTAQGGTHVNGLRQGLLDAMREFCEFRNLLPRGLKLAPEDIWERIAFVLSMKMQEPQFSG
QTKERLSSREAAAFVSGVVKDAFSLWLNAHPELGMQLAELAINNAGRRLKAGKKVERKKI
TQGPALPGKLADCAGQDPMRAELFLVEGDSAGGSAKQARDKEFQAIMPLRGKILNTWEVD
GGEVLASQEVHDIAVAIGVDPGAADLGQLRYGKICILADADSDGLHIATLLCALFVQHFR
PLVEAGHVYVAMPPLYRIDLGKDIFYALDEAERDGILERLVAEKRRGKPQVTRFKGLGEM
NPPQLRETTMDPNTRRLVQLTLDDFEGTREIMDMLLAKKRAGDRKSWLESKGNLAEVLV