Protein Info for Psest_0477 in Pseudomonas stutzeri RCH2

Annotation: thiamine biosynthesis protein ThiC

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF13667: ThiC-associated
  • amino acids 21 to 98 (78 residues), 116 bits, E=5.4e-38
• TIGR00190: phosphomethylpyrimidine synthase
  • amino acids 137 to 589 (453 residues), 724.1 bits, E=2.4e-222
• PF01964: ThiC_Rad_SAM
  • amino acids 139 to 587 (449 residues), 617.2 bits, E=1.4e-189

Best Hits

Swiss-Prot: 98% identical to THIC_PSEU5: Phosphomethylpyrimidine synthase (thiC) from Pseudomonas stutzeri (strain A1501)

KEGG orthology group: K03147, thiamine biosynthesis protein ThiC (inferred from 98% identity to psa:PST_3816)

MetaCyc: 73% identical to phosphomethylpyrimidine synthase (Escherichia coli K-12 substr. MG1655)
PYRIMSYN1-RXN [EC: 4.1.99.17]

Predicted SEED Role

"Hydroxymethylpyrimidine phosphate synthase ThiC (EC 4.1.99.17)" (EC 4.1.99.17)

MetaCyc Pathways

• superpathway of thiamine diphosphate biosynthesis II (9/11 steps found)
• superpathway of thiamine diphosphate biosynthesis I (8/10 steps found)
• 4-amino-2-methyl-5-diphosphomethylpyrimidine biosynthesis I (2/2 steps found)
• superpathway of thiamine diphosphate biosynthesis III (eukaryotes) (3/7 steps found)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.1.99.17

Sequence Analysis Tools

See L0GIF5 at UniProt or InterPro

Protein Sequence (626 amino acids)

>Psest_0477 thiamine biosynthesis protein ThiC (Pseudomonas stutzeri RCH2)
MRVEQQNLSESAQVDQQSIQPFPRSQKIYVQGSRPDIRVPMREISLDVTPTDFGGEINAP
VTVYDTSGPYTDPNVQIDVRKGLADVRSAWIEERGDTEKLPGLTSEFGQRRLNDAELTAM
RFAHVRHPRRAKAGHNVSQMHYARKGIITPEMEYVAIRENMKLTEAREAGLLKDQHAGHS
FGASIPKEITPEFVRDEVARGRAIIPANINHTELEPMIIGRNFLVKINGNIGNSALGSSI
EEEVAKLTWGIRWGSDTVMDLSTGKHIHETREWIIRNSPVPIGTVPIYQALEKVNGVAED
LTWELFRDTLIEQAEQGVDYFTIHAGVLLRYVPLTAKRVTGIVSRGGSIMAKWCLAHHKE
NFLYTNFEEICEIMKAYDVSFSLGDGLRPGSIADANDAAQFGELETLGELTKIAWKHDVQ
CMIEGPGHVPMHMIKENMDKQLECCDEAPFYTLGPLTTDIAPGYDHITSGIGAAMIGWFG
CAMLCYVTPKEHLGLPNKDDVKTGIITYKIAAHAADLAKGHPGAQIRDNALSKARFEFRW
EDQFNLGLDPDTARAFHDETLPKESAKVAHFCSMCGPKFCSMKITQEVREYAAEHGLTDE
QKAIEAGFAEQSSRFKDEGSVIYKQV