Protein Info for Psest_0476 in Pseudomonas stutzeri RCH2

Annotation: type I secretion outer membrane protein, TolC family

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 484 signal peptide" amino acids 1 to 22 (22 residues), see Phobius details TIGR01844: type I secretion outer membrane protein, TolC family" amino acids 30 to 458 (429 residues), 422.1 bits, see alignment E=1.2e-130 PF02321: OEP" amino acids 36 to 220 (185 residues), 95.8 bits, see alignment E=1.5e-31 amino acids 246 to 443 (198 residues), 105.8 bits, see alignment E=1.3e-34

Best Hits

KEGG orthology group: K12340, outer membrane channel protein TolC (inferred from 98% identity to psa:PST_3817)

Predicted SEED Role

"Type I secretion outer membrane protein, TolC precursor" in subsystem Multidrug Resistance Efflux Pumps or Ton and Tol transport systems

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GED1 at UniProt or InterPro

Protein Sequence (484 amino acids)

>Psest_0476 type I secretion outer membrane protein, TolC family (Pseudomonas stutzeri RCH2)
MLRRLPLALAVASLTAGAAWAQETVPLASKTDLVSVYQQAVNNNADLAAARAQFRARQEI
VPQARAGLLPNLSAGAELSDTETDVDTSRGSTSLSRSGTAYQATLSQPIFRADRWFQLKA
AQAVSEQAAIEYSIAEQDLILQSAQSYFAVLRAQDNLAAVKAEEAAFKRQLDQANERFEV
GLSDKTDVLEAQAGFDTARANRSIAQRQVDDAFQALFTLTNREYIALEGIRHTLPVLAPA
PNEAKAWVDTATRQNLNLLAVNYAVTAAEETLRQRRSGHAPTLDAVASYRKGDNDSLGFS
NSQNIPGFDMPRYTGDVEQRSIGLQLNIPLYSGGLTTSQSREAYYQLSQSEQQRESLRRQ
VVESTRNLHRAVNTDIEQVEARRQSIISNQSALEATEIGYQVGTRNIVDVLDTQRRLYGA
VRDYNNARYDYILDNLRLKQAAGTLNPDDLQQLAAYLKPDYNPDTDFLPPDLPQTIGQPV
RVDY