Protein Info for Psest_0474 in Pseudomonas stutzeri RCH2

Annotation: Membrane transporters of cations and cationic drugs

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 132 transmembrane" amino acids 6 to 24 (19 residues), see Phobius details amino acids 31 to 51 (21 residues), see Phobius details amino acids 57 to 81 (25 residues), see Phobius details PF00893: Multi_Drug_Res" amino acids 3 to 78 (76 residues), 81.7 bits, see alignment E=2.3e-27

Best Hits

Predicted SEED Role

"Ethidium bromide-methyl viologen resistance protein EmrE"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GGF6 at UniProt or InterPro

Protein Sequence (132 amino acids)

>Psest_0474 Membrane transporters of cations and cationic drugs (Pseudomonas stutzeri RCH2)
MSGYVYLAIAICAEVVATTSMKALAGFSRPLPLLLVVTGYSLSFWMLALVVKTIPVGIAY
AIWAGLGIVLVSIAAAVLSPVSGPAGSTRYGPDHRWRGGHSAVLRQHRPLSTPRTACSYT
ARPFPRMRSAHA