Protein Info for PGA1_c04590 in Phaeobacter inhibens DSM 17395

Annotation: putative threonine dehydratase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 43% identical to LTHAD_PSESP: L-threo-3-hydroxyaspartate ammonia-lyase (thadh) from Pseudomonas sp.

KEGG orthology group: K01754, threonine dehydratase [EC: 4.3.1.19] (inferred from 72% identity to sit:TM1040_3568)

MetaCyc: 43% identical to L-threo-3-hydroxy aspartate ammonia-lyase (Pseudomonas sp. T62)
Threo-3-hydroxyaspartate ammonia-lyase. [EC: 4.3.1.16]

Predicted SEED Role

"Threonine dehydratase, catabolic (EC 4.3.1.19)" in subsystem Branched-Chain Amino Acid Biosynthesis or Glycine and Serine Utilization or Threonine anaerobic catabolism gene cluster or Threonine degradation (EC 4.3.1.19)

MetaCyc Pathways

superpathway of branched chain amino acid biosynthesis (17/17 steps found)
superpathway of L-isoleucine biosynthesis I (12/13 steps found)
superpathway of L-threonine metabolism (15/18 steps found)
L-isoleucine biosynthesis I (from threonine) (7/7 steps found)
L-methionine degradation II (3/3 steps found)
L-threonine degradation I (5/6 steps found)
L-threonine degradation V (1/2 steps found)
hypoglycin biosynthesis (4/14 steps found)
cyclosporin A biosynthesis (2/15 steps found)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.3.1.19

Use Curated BLAST to search for 4.3.1.16 or 4.3.1.19

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See I7EJ66 at UniProt or InterPro

Protein Sequence (324 amino acids)

>PGA1_c04590 putative threonine dehydratase (Phaeobacter inhibens DSM 17395)
MTSFAAIAAAADRLSGHARETPLLSSPFLDEIAGRRVLIKAECLQHTGSFKFRGGWAALS
ALTDEQRHRGVLAYSSGNHAQGVAAAAAAHTTPAVILMPQDAPAIKIANTRALGAEVVLF
DRAKDNRDAIGAELAEARGLTLIKPYDDVEVVAGQGTVGLEIARQAADLDVTEADVLVCC
GGGGLTAGVALALEQTAPKMKVSPCEPERFDDVTRSLVAGKICRNEASAGSLCDAILTPA
PGEITFPILARLCGSGIVVSEEDAMRAMVLAFLRLKIVLEPGGAVSLAAALFHGDQLSGP
AAIAIATGGNVDAALFTEALHRFG