Protein Info for Psest_0449 in Pseudomonas stutzeri RCH2

Annotation: Glutamine synthetase adenylyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 950 981 signal peptide" amino acids 1 to 21 (21 residues), see Phobius details PF03710: GlnE" amino acids 50 to 297 (248 residues), 301.9 bits, see alignment E=3.5e-94 amino acids 575 to 829 (255 residues), 312.9 bits, see alignment E=1.5e-97 PF08335: GlnD_UR_UTase" amino acids 317 to 455 (139 residues), 102.3 bits, see alignment E=2.4e-33 amino acids 851 to 949 (99 residues), 38.2 bits, see alignment E=1.4e-13

Best Hits

Swiss-Prot: 76% identical to GLNE_PSESM: Bifunctional glutamine synthetase adenylyltransferase/adenylyl-removing enzyme (glnE) from Pseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000)

KEGG orthology group: K00982, glutamate-ammonia-ligase adenylyltransferase [EC: 2.7.7.42] (inferred from 93% identity to psa:PST_3851)

Predicted SEED Role

"Glutamate-ammonia-ligase adenylyltransferase (EC 2.7.7.42)" in subsystem Ammonia assimilation (EC 2.7.7.42)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.7.42

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GGD3 at UniProt or InterPro

Protein Sequence (981 amino acids)

>Psest_0449 Glutamine synthetase adenylyltransferase (Pseudomonas stutzeri RCH2)
MSLPPLVALPSSLLPFVSRAEESFLAAAGSLSEALAARCHVWLAEQREAFSRVCAASDFV
SEQVSRDPQMLLQLAELGWLERSLEAGEMREALGELVGACDNEDALALTLRRFRNRQQVR
IIWRDLTRRADLAETCRDLSDLADASVDLAYHWLYVRHCEQFGVPTGRRSGTAQHMVILG
MGKLGAHELNLSSDIDLIFGYPEGGETVGAKRSLDNQEFFVRLGQRLIKALDAITADGFA
FRVDMRLRPYGSSGPLVYSFNALEQYYQDQGRDWERYAMIKARVIGGDQQAGKELLEMLR
PFVYRRYLDFSAIEALRTMKQLIQQEVRRKGMAANIKLGAGGIREVEFIAQAFQLIHGGR
DLSLQQRPLLGVLATLEGQSYLPAAAVVELREGYEFLRYTEHALQAIADRQTQMLPESEV
DCARVAFMLGFDSWLAFHERLLHWRGRIDWHFHQVIADPDEDEAAEGDTLVGGEWLPLWE
QDWDEEFACRQLSEAGFRDGQGACQRLAALRNASQVRTMQRLGRERLDAFIPRLLAQTVE
QADPDLVLERVLPLVEAVARRSAYLVLLTENPGALQRLLELCAASPWIAEQIARFPLLLD
ELLNAGRLYSPPLAPELAAELRERLMRIPEDDLEQQMEALRHFKLAHRLRVAASEIAGTL
PLMKVSDYLTWLAEAILQEVLTLAWRHTVARHGQPQRSDGTLCDPAFVIVGYGKVGGIEL
GHGSDLDLVFIHDGDPAAETNGAKPIDTAQFFTRLGQRIIHLLTTQTTSGQLYEVDMRLR
PSGASGLLVSSLGAFERYQSQEAWTWEHQALVRARVLVGCPQLTTDFERVRAAVLGRERD
LDALRREVSDMRAKMRDNLGTRATHAGTAEQAFDGAAEFNLKQDAGGIVDIEFMVQYAAL
AWSHQHPELLRYTDNIRILDGLEQAGLMTGDEVRLLQDAYKAYRAAAHRQSLQKQPGLVS
GDQFHDERRAVMRIWRELGLS