Protein Info for Psest_0447 in Pseudomonas stutzeri RCH2

Annotation: pyruvate dehydrogenase complex dihydrolipoamide acetyltransferase, long form

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00364: Biotin_lipoyl
  • amino acids 4 to 74 (71 residues), 76.9 bits, E=4.3e-25
  • amino acids 126 to 197 (72 residues), 80.2 bits, E=4.1e-26
  • amino acids 241 to 312 (72 residues), 79.1 bits, E=8.6e-26
• TIGR01348: dihydrolipoyllysine-residue acetyltransferase
  • amino acids 230 to 667 (438 residues), 668.5 bits, E=4.3e-205
• PF02817: E3_binding
  • amino acids 366 to 401 (36 residues), 55.8 bits, 2e-18
• PF00198: 2-oxoacid_dh
  • amino acids 438 to 667 (230 residues), 263.9 bits, E=5.9e-82

Best Hits

Swiss-Prot: 70% identical to ODP2_AZOVI: Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex from Azotobacter vinelandii

KEGG orthology group: K00627, pyruvate dehydrogenase E2 component (dihydrolipoamide acetyltransferase) [EC: 2.3.1.12] (inferred from 75% identity to pfo:Pfl01_0462)

Predicted SEED Role

"Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex (EC 2.3.1.12)" in subsystem Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate (EC 2.3.1.12)

MetaCyc Pathways

• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (23/26 steps found)
• superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (18/22 steps found)
• pyruvate fermentation to acetate and (S)-lactate I (4/4 steps found)
• pyruvate decarboxylation to acetyl CoA I (3/3 steps found)
• pyruvate fermentation to acetate II (3/3 steps found)
• pyruvate fermentation to acetate V (3/3 steps found)
• photosynthetic 3-hydroxybutanoate biosynthesis (engineered) (19/26 steps found)
• 1-butanol autotrophic biosynthesis (engineered) (19/27 steps found)
• anaerobic energy metabolism (invertebrates, mitochondrial) (5/10 steps found)
• superpathway of anaerobic energy metabolism (invertebrates) (9/17 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• Citrate cycle (TCA cycle)
• Glycolysis / Gluconeogenesis
• Pyruvate metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.3.1.12

Use Curated BLAST to search for 2.3.1.12

Sequence Analysis Tools

See L0GIC7 at UniProt or InterPro

Protein Sequence (667 amino acids)

>Psest_0447 pyruvate dehydrogenase complex dihydrolipoamide acetyltransferase, long form (Pseudomonas stutzeri RCH2)
MSETIRVPDIGSGEGEVIELFVKVGDRIEADQSILTLESDKASMEIPAPKAGVVKSLKVK
LGDRLKEGDELLELESEDGQDSAAPSQAAAEPAGAASGGPTDETEAPTPPGDDDASASAG
EGETQEIKVPDIGSLGKASVIEIAVKPGDTIAAEQALITLESDKASMEIPSPAAGVVESI
SVKVGDEVGTGDLILVLKGAAPSKPAASKAPASQAASAPKQKLSEPAVEEPAEDAGESVE
EVRIPDIGSSGSANVIEVMVKAGDSVEADQSLITLESDKASMEIPAPKAGIVESLSIKVG
DEARTGDLILTLRVQGTAPAKKPATKAEAAPQQQAVAPNKQGVPEAKAAATPAPAVSGPS
KAGTKVHAGPAVRMLAREFGVELADVPATGPKGRILKEDVQAYVKNMLHKVKQAPAEGAT
GGAGIPPIPAIDFSRFGEVEEVAMTRLMQVGAANLHRSWLNVPHVTQFESADITELEAFR
VAQKAIAEKAGVKLTVLPLLLKACAHLLKELPDFNSSLAPSGKALIRKKYVHVGFAVDTP
DGLMVPVIRNVDQKSLLQLAGEAAELAEKARTKKLSPDAMQGACFTISSLGHIGGTGFTP
IVNAPEVAILGVSKATMQPVWDGKAFQPRLMLPLSLSYDHRVINGAAAARFTKRLSELLA
DIRTMLL