Protein Info for Psest_0443 in Pseudomonas stutzeri RCH2

Annotation: methionine-S-sulfoxide reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 181 signal peptide" amino acids 1 to 22 (22 residues), see Phobius details TIGR00401: peptide-methionine (S)-S-oxide reductase" amino acids 4 to 157 (154 residues), 199 bits, see alignment E=2.7e-63 PF01625: PMSR" amino acids 5 to 158 (154 residues), 213.4 bits, see alignment E=9.5e-68

Best Hits

Swiss-Prot: 54% identical to MSRA_TRIEI: Peptide methionine sulfoxide reductase MsrA (msrA) from Trichodesmium erythraeum (strain IMS101)

KEGG orthology group: K07304, peptide-methionine (S)-S-oxide reductase [EC: 1.8.4.11] (inferred from 54% identity to chl:Chy400_1802)

Predicted SEED Role

"Peptide methionine sulfoxide reductase MsrA (EC 1.8.4.11)" (EC 1.8.4.11)

Isozymes

Compare fitness of predicted isozymes for: 1.8.4.11

Use Curated BLAST to search for 1.8.4.11

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GI33 at UniProt or InterPro

Protein Sequence (181 amino acids)

>Psest_0443 methionine-S-sulfoxide reductase (Pseudomonas stutzeri RCH2)
MKTEKIVLAGGCFWCLEPVFKRIKGVQSVKCGYTGGTLNAPTYDDICTGKTGHAEAIKIE
FDSSKISLSELLEIFFAIHDPTTLNRQGADIGTQYRSAIFYDSDEQLVTAKTAILETEKN
GLWKNPTATTLERLTEFFEAEEYHQDFFTKNPSNRYCQISIAPKLAKLETIFKNQKATGR
E