Protein Info for HP15_p187g66 in Marinobacter adhaerens HP15

Annotation: UDP-glucose 4-epimerase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01370: Epimerase
  • amino acids 23 to 268 (246 residues), 203.2 bits, E=1.3e-63
• PF02719: Polysacc_synt_2
  • amino acids 24 to 287 (264 residues), 74.2 bits, E=3.3e-24
• PF16363: GDP_Man_Dehyd
  • amino acids 24 to 335 (312 residues), 170.6 bits, E=2e-53
• PF01073: 3Beta_HSD
  • amino acids 24 to 257 (234 residues), 71.8 bits, E=1.5e-23
• PF07993: NAD_binding_4
  • amino acids 25 to 172 (148 residues), 39.5 bits, E=1.1e-13
• PF13460: NAD_binding_10
  • amino acids 27 to 176 (150 residues), 31 bits, E=6.7e-11

Best Hits

Swiss-Prot: 70% identical to VIPB_SALTI: Vi polysaccharide biosynthesis protein VipB/TviC (vipB) from Salmonella typhi

KEGG orthology group: K01784, UDP-glucose 4-epimerase [EC: 5.1.3.2] (inferred from 80% identity to abb:ABBFA_003457)

MetaCyc: 74% identical to UDP-N-acetylglucosamine C4-epimerase subunit (Pseudomonas aeruginosa)
UDP-N-acetylglucosamine 4-epimerase. [EC: 5.1.3.7]; 5.1.3.- [EC: 5.1.3.7]

Predicted SEED Role

"UDP-glucose 4-epimerase (EC 5.1.3.2)" in subsystem Lacto-N-Biose I and Galacto-N-Biose Metabolic Pathway or Lactose and Galactose Uptake and Utilization or N-linked Glycosylation in Bacteria or Rhamnose containing glycans or linker unit-arabinogalactan synthesis (EC 5.1.3.2)

MetaCyc Pathways

• UDP-N-acetyl-α-D-galactosaminuronate biosynthesis (2/2 steps found)
• UDP-α-D-galactose biosynthesis (1/1 steps found)
• UDP-N-acetyl-D-galactosamine biosynthesis I (1/1 steps found)
• colanic acid building blocks biosynthesis (8/11 steps found)
• superpathway of UDP-glucose-derived O-antigen building blocks biosynthesis (4/6 steps found)
• UDP-α-D-galactofuranose biosynthesis (1/2 steps found)
• UDP-N-acetyl-D-galactosamine biosynthesis II (4/7 steps found)
• D-galactose detoxification (1/3 steps found)
• D-galactose degradation I (Leloir pathway) (2/5 steps found)
• stachyose degradation (2/7 steps found)
• UDP-sugars interconversion (3/9 steps found)
• teichuronic acid biosynthesis (B. subtilis 168) (2/9 steps found)
• superpathway of UDP-N-acetylglucosamine-derived O-antigen building blocks biosynthesis (10/24 steps found)
• superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis (12/33 steps found)
• mycolyl-arabinogalactan-peptidoglycan complex biosynthesis (1/18 steps found)

KEGG Metabolic Maps

• Aminosugars metabolism
• Galactose metabolism
• Nucleotide sugars metabolism

Isozymes

Compare fitness of predicted isozymes for: 5.1.3.2

Use Curated BLAST to search for 5.1.3.2 or 5.1.3.7

Sequence Analysis Tools

See E4PS28 at UniProt or InterPro

Protein Sequence (343 amino acids)

>HP15_p187g66 UDP-glucose 4-epimerase (Marinobacter adhaerens HP15)
MLSADNRRYPQLLDQLPSQPKTWLITGAAGFIGSNLLEHLLKLNQHVIALDNFATGHQSN
LDEVRSLVSTEQWTRLQSIEGDIRNPEDCARACEGVDYVLHQAALGSVPRSLNDPITTNA
ANITGFLNMLVAARDAGVKSFTYAASSSTYGDHPALPKVEEKIGKPLSPYAVTKHVNELY
AEVFARSYGFKAIGLRYFNVFGKRQDPNGAYAAVIPKWTAAMVRGEDVFINGDGETSRDF
CFIENAVQANLLAATAEDSARNEVYNVAVGDRTTLNDLFAALKSALAENGVVYDKAPVYR
DFRPGDVRHSQADIGKASSRLGYNPEFRIVEGIAKAMPWYLKH