Protein Info for Psest_4360 in Pseudomonas stutzeri RCH2

Annotation: F0F1-type ATP synthase, subunit a

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 284 transmembrane" amino acids 54 to 74 (21 residues), see Phobius details amino acids 113 to 132 (20 residues), see Phobius details amino acids 138 to 155 (18 residues), see Phobius details amino acids 162 to 179 (18 residues), see Phobius details amino acids 198 to 215 (18 residues), see Phobius details amino acids 227 to 247 (21 residues), see Phobius details amino acids 253 to 276 (24 residues), see Phobius details TIGR01131: ATP synthase F0, A subunit" amino acids 52 to 279 (228 residues), 140 bits, see alignment E=5.5e-45 PF00119: ATP-synt_A" amino acids 62 to 277 (216 residues), 185.2 bits, see alignment E=7.8e-59

Best Hits

Swiss-Prot: 96% identical to ATP6_PSEU5: ATP synthase subunit a (atpB) from Pseudomonas stutzeri (strain A1501)

KEGG orthology group: K02108, F-type H+-transporting ATPase subunit a [EC: 3.6.3.14] (inferred from 96% identity to psa:PST_4197)

MetaCyc: 63% identical to ATP synthase Fo complex subunit a (Escherichia coli K-12 substr. MG1655)
ATPSYN-RXN [EC: 7.1.2.2]; RXN0-7041 [EC: 7.1.2.2]

Predicted SEED Role

"ATP synthase F0 sector subunit a"

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.14

Use Curated BLAST to search for 3.6.3.14 or 7.1.2.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GPZ0 at UniProt or InterPro

Protein Sequence (284 amino acids)

>Psest_4360 F0F1-type ATP synthase, subunit a (Pseudomonas stutzeri RCH2)
MASTPAEYIQHHLQNLTYGKLPAGYERADGSVLDQATWTIAQTGLEARDMGFMAFHLDTL
GWSLLMGAIFILLFRSAAKSATAGVPGKLQNFVEMCVEFVEGVVKDTFHGRNTLIAPLAL
TIFVWVFLMNSLKWIPVDYIPGLASLLGLPAFKIVPTADPNGTFGLSLGVFILILFYSVK
VKGFGGFSKELAFTPFNHWSLVPFNLFLEILGLLTKPLSLALRLFGNMYAGEVVFILIAL
LPFYVQWTLNVPWAIFHILVIPLQAFIFMVLTVVYLSSAHEEHH