Protein Info for HP15_4174 in Marinobacter adhaerens HP15

Annotation: formate dehydrogenase, alpha subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 966 PF13510: Fer2_4" amino acids 28 to 103 (76 residues), 80.2 bits, see alignment 4.1e-26 PF00111: Fer2" amino acids 35 to 93 (59 residues), 24.4 bits, see alignment 1e-08 PF10588: NADH-G_4Fe-4S_3" amino acids 110 to 149 (40 residues), 62.4 bits, see alignment (E = 1e-20) PF12838: Fer4_7" amino acids 175 to 231 (57 residues), 31.5 bits, see alignment 8.9e-11 PF00037: Fer4" amino acids 216 to 231 (16 residues), 22.9 bits, see alignment (E = 2.5e-08) PF04879: Molybdop_Fe4S4" amino acids 247 to 297 (51 residues), 53 bits, see alignment 1.2e-17 TIGR01591: formate dehydrogenase, alpha subunit" amino acids 251 to 926 (676 residues), 911.7 bits, see alignment E=1.2e-278 PF00384: Molybdopterin" amino acids 303 to 734 (432 residues), 239.7 bits, see alignment E=2.2e-74 PF01568: Molydop_binding" amino acids 818 to 923 (106 residues), 94.5 bits, see alignment E=1.8e-30

Best Hits

KEGG orthology group: K00123, formate dehydrogenase, alpha subunit [EC: 1.2.1.2] (inferred from 73% identity to bcj:BCAL2978)

Predicted SEED Role

"NAD-dependent formate dehydrogenase alpha subunit" in subsystem Formate hydrogenase

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.2.1.2

Use Curated BLAST to search for 1.2.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See E4PMN0 at UniProt or InterPro

Protein Sequence (966 amino acids)

>HP15_4174 formate dehydrogenase, alpha subunit (Marinobacter adhaerens HP15)
MLQYFDPDTSGLNPNIDYGTPPSNSETLVSLSIDGTDIAVPEGTSVLRAATLAGINIPKL
CASDNLEAFGSCRMCAVEIEGRRGYPASCTTPVSEGMTVTTQNAKLAKLRRNIMELYISD
HPLDCLTCPANGNCELQDVAGAVGLREVRYGFEGENHLDVEVDDSNPYFSFDPSKCIVCS
RCIRACEEVQGTFALTIDGRGFDSKVSAGQNDPFMDSECVSCGACVQACPTSTLMEKSVI
DAGQPEHSVVTTCAYCGVGCSFKAEMKGDQLVRMVPYKGGDANQGHSCVKGRFAFGYATH
KDRIKEPMIRESIDDPWQVVTWEEAIEFSAKKLKDTQARYGRESIGGITSSRCTNEETYL
VQKLIRAAFGNNNTDTCARVCHSPTGFGLKTTMGESAGTQTFDSVMKADTIVVIGANPTD
AHPVFASQMRKRLRQGAKLIVIDPRRIDLLKTPHGTEGIHLPLRPGTNVAMINSLAHVIV
TEGLEDKGFIESRCELKQYNQWRDFIAEERHSPEALEVVTGVSADLVRQAARAYGEAHNG
AIYYGLGVTEHSQGSTMVMGIANLAMATGNIGREGVGVNPLRGQNNVQGSCDMGSFPHEL
PGYQHVGDSAVRTKFQDAWNVVIDNEPGLRIPNMFDAAIAGSFKAMYVQGEDIAQSDPNT
QHVEAALSSLDCLIVQDIFLNETAKYAHVLLPGSTFLEKNGTFTNAERRINRVRKVMAPV
AGMEDWEVTMALSNALGYPMHYNHPSEIMDEIARLTPTFTGVSYDRLDDLGSIQWPCNDE
HPDGTPTMHTLDFPIGKGKFAVTEYVATEEKTNRKFPLLLTTGRILSQYNVGAQTRRTEN
SQWHSEDVLEIHPSDAELRGVREGDWLGVSSRVGQTVLRARISDRMLPGVVWTTFHHPGS
GANVITTDSSDWATNCPEYKVTAVQVEKVSQPSAWQRHFAQFDERQHQLLDSKAGEEERS
DVASFE