Protein Info for Psest_4281 in Pseudomonas stutzeri RCH2

Annotation: ABC-type spermidine/putrescine transport system, permease component I

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 298 transmembrane" amino acids 12 to 33 (22 residues), see Phobius details amino acids 78 to 103 (26 residues), see Phobius details amino acids 115 to 136 (22 residues), see Phobius details amino acids 166 to 186 (21 residues), see Phobius details amino acids 221 to 240 (20 residues), see Phobius details amino acids 265 to 286 (22 residues), see Phobius details PF00528: BPD_transp_1" amino acids 96 to 284 (189 residues), 50.1 bits, see alignment E=1.4e-17

Best Hits

Swiss-Prot: 60% identical to POTH_ECOLI: Putrescine transport system permease protein PotH (potH) from Escherichia coli (strain K12)

KEGG orthology group: K11075, putrescine transport system permease protein (inferred from 96% identity to psa:PST_0070)

MetaCyc: 60% identical to putrescine ABC transporter membrane subunit PotH (Escherichia coli K-12 substr. MG1655)
ABC-25-RXN [EC: 7.6.2.11, 7.6.2.16]

Predicted SEED Role

"Putrescine transport system permease protein PotH (TC 3.A.1.11.2)" in subsystem Polyamine Metabolism (TC 3.A.1.11.2)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.6.2.11 or 7.6.2.16

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GTP3 at UniProt or InterPro

Protein Sequence (298 amino acids)

>Psest_4281 ABC-type spermidine/putrescine transport system, permease component I (Pseudomonas stutzeri RCH2)
MKRSPLNSGRRLVIGVPFLWLFLFFLLPFAIVLKISFAEADVAIPPYTDIFAYADQQLQV
LINLGNYIFLSEDELYLAAYLGSLRIAFFSTLLCLLIGYPMAYAIARARKDLQTVFLLLI
MMPTWTAILIRVYAWMGILSSNGLLNSLLLGMGLIDTPLQILNTDIAVYIGVVYSYLPFM
ILPLYANLVKHDPSLLEAASDLGARNLTSFWKITVPLSKNGIIAGCMLVFIPVVGEFVIP
ELLGGPETLMIGKVLWQEFFNNRDWPVASALAVVMLAILLIPIILFNRNQAKELEGKL