Protein Info for Psest_4280 in Pseudomonas stutzeri RCH2

Annotation: ABC-type spermidine/putrescine transport system, permease component II

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 transmembrane" amino acids 9 to 30 (22 residues), see Phobius details amino acids 66 to 85 (20 residues), see Phobius details amino acids 98 to 124 (27 residues), see Phobius details amino acids 136 to 158 (23 residues), see Phobius details amino acids 179 to 202 (24 residues), see Phobius details amino acids 239 to 258 (20 residues), see Phobius details PF00528: BPD_transp_1" amino acids 75 to 263 (189 residues), 46.6 bits, see alignment E=1.7e-16

Best Hits

Swiss-Prot: 64% identical to POTI_ECOL6: Putrescine transport system permease protein PotI (potI) from Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)

KEGG orthology group: K11074, putrescine transport system permease protein (inferred from 97% identity to psa:PST_0071)

MetaCyc: 64% identical to putrescine ABC transporter membrane subunit PotI (Escherichia coli K-12 substr. MG1655)
ABC-25-RXN [EC: 7.6.2.11, 7.6.2.16]

Predicted SEED Role

"Putrescine transport system permease protein PotI (TC 3.A.1.11.2)" in subsystem Polyamine Metabolism (TC 3.A.1.11.2)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.6.2.11 or 7.6.2.16

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GTX4 at UniProt or InterPro

Protein Sequence (300 amino acids)

>Psest_4280 ABC-type spermidine/putrescine transport system, permease component II (Pseudomonas stutzeri RCH2)
MRRWSFSNLILVLGLLFIYLPMVILVIYSFNASRLVTVWGGWSLKWYAGLLDNSQLMGAV
MRSLEVAAYTAVAAVALGTMAAFVLTRIPVFRGRTLFGGLVTAPLVMPEVITGLSLLLLF
VAMAQLVGWPAQRGLVTIWIAHTTFCSAYVAVVVMARLRELDLSIEEAAMDLGARPWKVF
FLITMPMIAPSLAAGGMLSFALSLDDLVLASFVSGPGSTTLPMEIFSAVRLGVKPEINAV
ASLILLSVSLATFLAWYLTRRAEKRRVRAMQQAMDETTGKSTWRQVIDSRKVLIPPSAHS