Protein Info for Psest_4259 in Pseudomonas stutzeri RCH2

Annotation: Multidrug resistance efflux pump

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 358 signal peptide" amino acids 1 to 29 (29 residues), see Phobius details PF16576: HlyD_D23" amino acids 48 to 301 (254 residues), 32.9 bits, see alignment E=6.1e-12 PF13533: Biotin_lipoyl_2" amino acids 53 to 97 (45 residues), 46.6 bits, see alignment 3.4e-16 PF13437: HlyD_3" amino acids 224 to 322 (99 residues), 42 bits, see alignment E=2.1e-14

Best Hits

Swiss-Prot: 56% identical to YHII_ECOLI: Uncharacterized protein YhiI (yhiI) from Escherichia coli (strain K12)

KEGG orthology group: K01993, HlyD family secretion protein (inferred from 97% identity to psa:PST_0087)

Predicted SEED Role

"HlyD family secretion protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GPP5 at UniProt or InterPro

Protein Sequence (358 amino acids)

>Psest_4259 Multidrug resistance efflux pump (Pseudomonas stutzeri RCH2)
MKAPAQKTLRRVLFVLVALVVIGLLAWSELRTDGLGEGFASGNGRIEATEIDVATKLGGR
IREISVDEGDFVQPGQVIARMDTEVLDAQLNQARAQVRQAENAILTAQALVTQRESEKAT
AEAVVLQRRAELTAAQKRHQRTETLVGRNALPRQQLDDDLAAMQSAQAALAASRSQVLSA
DAGIAAARSQVIEAQSALEAAQASVVRLQADITDSELKTDRVARVQYRVAQPGEVLGAGG
KLLNLVDLADVYMTFFLPERQAGRVAMGSEVRLVIDAAPQYVIPAKVTYVASVAQFTPKT
VETESEREKLMFRVKARIDPELLRKHMEQVKTGLPGMAYLKLDADAEWPAHLQINVGK