Protein Info for Psest_4246 in Pseudomonas stutzeri RCH2

Annotation: type VI secretion protein IcmF

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1100 1179 transmembrane" amino acids 16 to 38 (23 residues), see Phobius details amino acids 50 to 70 (21 residues), see Phobius details amino acids 448 to 467 (20 residues), see Phobius details TIGR03348: type VI secretion protein IcmF" amino acids 18 to 1176 (1159 residues), 1153.7 bits, see alignment E=0 PF14331: IcmF-related_N" amino acids 200 to 452 (253 residues), 263.6 bits, see alignment E=3.6e-82 PF06761: IcmF-related" amino acids 502 to 793 (292 residues), 270.8 bits, see alignment E=3.7e-84 PF21070: IcmF_helical" amino acids 947 to 1045 (99 residues), 28.8 bits, see alignment E=2.4e-10 PF06744: IcmF_C" amino acids 1054 to 1159 (106 residues), 89.9 bits, see alignment E=2.3e-29

Best Hits

KEGG orthology group: K11891, type VI secretion system protein ImpL (inferred from 80% identity to pmy:Pmen_0104)

Predicted SEED Role

"IcmF-related protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GTK4 at UniProt or InterPro

Protein Sequence (1179 amino acids)

>Psest_4246 type VI secretion protein IcmF (Pseudomonas stutzeri RCH2)
MKTFFGKLGAVLRRTWVWSLLLVLLLAVVVWFVGPLLAVDDYRFWEASTARLLSISVLFL
GWGLAMVFTSWRASARRKRDAEDQDVQEQLRRDGLIGEEQQELRHRFKQALRTLKTSSLY
RGRSEKWRSELPWYLLIGPQGSGKTSLLDFSGLDFPLNRSEQQRLTKDVSGTRYADWYFA
DHAVLIDTAGRYLTQPDAAVDGSVWDTLLGLLRKRRARPLNGVLVNLPVDQLQGGSELEL
ENLARQTRQRLHEIHQRLNVDVPVYLVLSKADRILGFDDFFEQLSREESDQVLGASFRKE
QNGSDAQVVRQEFEELLRRLNSQVILRMHQERDTQRRGRILDFPHQLGQIGERLSLFIEL
AFAGNRYQRASQLRGFYLTSAPQLRETLDPLTAGIGRQLGLSSSALPTFRSGRARFINQL
LSRVIFPEAELAGLDQREVRRIDWGQRALYAGAFGCLLLFGAAWALNFSGNHERLEQLRD
IAQRLGSERKTIEAGDEALRTLKALDASYAATQVFPPKDEVSWLRRGGLYQGEAVDPTLH
EAYRRDLENLLLPRVARQLEAQIRANLNDREQLLGSLRAYLMLNLAERREAAFLKDWLAA
DWSLRHSGNAVAQQGLNTHFARLLEESFAAYQLNDNLVAKARAQLRSESLAAVVYRMLRD
QARNLPDYRLNTQLGPQASLFVGGDYTIPGFYTQRGYQKLFVAQGADLVSEILRDNWVLG
EGDSLSAKDLGRLLVEMEQLYFRDYAAHWSEAIAQLNILPAGSAAQGAAQLAGLTAANSP
LLQLLLEVRENTRFAGLAEATAGAGELAEAAQGAGGKLGKAAKLATAAAEQAQAALLKNT
PDTARKTLERRFAPLHQLLDENGAAGPELSASLQALDALQLQLASLAHASAPEQAAFEMA
KARMGGQRDAINQLRSSAARLPQPVGKWLGLLAEDSWSLVLTDAYHYLNQRYRNELYASY
RGSLRERYPFSAHSASDVALADFREFFKAQGLADSFFERYLKPFVSGSAGQYQLRRVDGR
GLPLSEHFLAQMSRAQTIRRSFFAENPNEPQILFKLEPYSLDSSLGRADFRFGNQQLEYR
HGPIVQTAFRWPAEADDGRTSLVVEELGGRRVGIEKNTGPWSLFRLIDLMQVDYHSGRDV
LMLKADLGGLRANYLLHAQRSPNPFDLALLRGFKLPATL