Protein Info for Psest_4233 in Pseudomonas stutzeri RCH2
Annotation: Acyl-CoA dehydrogenases
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: None (inferred from 94% identity to psa:PST_0101)MetaCyc: 47% identical to pimeloyl-CoA dehydrogenase small subunit (Rhodopseudomonas palustris)
Pimeloyl-CoA dehydrogenase. [EC: 1.3.1.62]
Predicted SEED Role
"Butyryl-CoA dehydrogenase (EC 1.3.99.2)" in subsystem Acetyl-CoA fermentation to Butyrate or Anaerobic respiratory reductases or Butanol Biosynthesis or Isobutyryl-CoA to Propionyl-CoA Module or Isoleucine degradation or Valine degradation (EC 1.3.99.2)
MetaCyc Pathways
- pyruvate fermentation to butanol II (engineered) (4/6 steps found)
- 1-butanol autotrophic biosynthesis (engineered) (19/27 steps found)
- pyruvate fermentation to hexanol (engineered) (7/11 steps found)
- benzoyl-CoA degradation III (anaerobic) (2/9 steps found)
KEGG Metabolic Maps
- Benzoate degradation via CoA ligation
- Butanoate metabolism
- Fatty acid metabolism
- Valine, leucine and isoleucine degradation
Isozymes
Compare fitness of predicted isozymes for: 1.3.99.2
Use Curated BLAST to search for 1.3.1.62 or 1.3.99.2
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See L0GPM5 at UniProt or InterPro
Protein Sequence (380 amino acids)
>Psest_4233 Acyl-CoA dehydrogenases (Pseudomonas stutzeri RCH2) MDFKLTEEQQMLQDTAARLVRDAYPFEQREKFSESELGFSAEFWAQLGELGLTAVPFAEE IGGFGGGGVETMLVMTELGRGLTLEPYLQSVIFAGGLLTQLGSDAQKEELLPQVAAGSLQ LAVALDEPQSHYNLNDVLTKAEAADGGYRLSGRKAVVIGGHSAGRIIVSARSAGDSRDEA GVSLFLVDPNAQGVSRRVYPTIDGRKGCELFLDNVQVGADALLGEIGNALPAIRYQQGRA IAAQCADALGSMDEACKLTLDYLKTRKQFGVPIGKFQVLQHRMVDMQTELEQATSMAILA ATFADGEDNDERSRIIAAAKYICARAARKVAEEAIQLHGGIGMTWEYNLAHHAKRLVMIA HQFGDDDHHLKAYAKLMQVA