Protein Info for Psest_4228 in Pseudomonas stutzeri RCH2

Annotation: Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 541 transmembrane" amino acids 244 to 259 (16 residues), see Phobius details PF00501: AMP-binding" amino acids 26 to 401 (376 residues), 308.7 bits, see alignment E=5.3e-96 PF13193: AMP-binding_C" amino acids 452 to 526 (75 residues), 72.8 bits, see alignment E=3.5e-24

Best Hits

KEGG orthology group: None (inferred from 88% identity to psa:PST_0106)

Predicted SEED Role

No annotation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GPM0 at UniProt or InterPro

Protein Sequence (541 amino acids)

>Psest_4228 Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II (Pseudomonas stutzeri RCH2)
MSASVQGYVAAELARGSYQNVHDLLSRACATHPQRTAFSCGDDHLTYAELGSRADAFARY
LRYHAGLQPGDRLALQLPNCLQYPIAVFGALKAGLVIVNTNPQYTAAEARHQFHDSGAKA
ILVLDRLLPQVRAVQADTALRQIILTSVDDLQQPIYESQEDGTLRFMQALRLGEQRSPVQ
REAGLDRLALLQYTGGTTGVSKGAMLTHRNLLANVLQTIELFDQPGLLEPEKDVRIAPLP
LYHIMAFATNCLSSVGMGLHTVFIRDGRNLDETIGAMQRHPFSLLSGINTLFVGLMNHPE
FRSIDFSHLKWATSGGAPLNSEVGRRWQALTGAPIREGFGLTEASPVVATGTQLSPYREG
YIGQALIETELRTVDDEGNDVPPETPGELWLRGPQVMQGYWQRPDETAKVLTPDGWLKTG
DIALLDVDGFVKIVDRKKDMILVSGFNVFPNEIEDVLMQHPSVRECVAVGVPDARKGEAV
KVFVSLKDDTVDERALLEHCRQYLTGYKMPSFLELRDELPKTAVGKLLRRQLRDEARAAA
S