Protein Info for Psest_4203 in Pseudomonas stutzeri RCH2

Annotation: NAD/NADP transhydrogenase alpha subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 373 PF05222: AlaDh_PNT_N" amino acids 4 to 136 (133 residues), 140.7 bits, see alignment E=3.7e-45 PF01262: AlaDh_PNT_C" amino acids 140 to 370 (231 residues), 236 bits, see alignment E=3.3e-74

Best Hits

Swiss-Prot: 52% identical to PNTAA_RHORU: NAD(P) transhydrogenase subunit alpha part 1 (pntAA) from Rhodospirillum rubrum

KEGG orthology group: K00324, NAD(P) transhydrogenase subunit alpha [EC: 1.6.1.2] (inferred from 100% identity to psa:PST_0124)

Predicted SEED Role

"NAD(P) transhydrogenase alpha subunit (EC 1.6.1.2)" in subsystem Phosphate metabolism (EC 1.6.1.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.6.1.2

Use Curated BLAST to search for 1.6.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GPJ7 at UniProt or InterPro

Protein Sequence (373 amino acids)

>Psest_4203 NAD/NADP transhydrogenase alpha subunit (Pseudomonas stutzeri RCH2)
MHIGVPLETHSGETRVAATPETVKKLIGQGHRVTLQSGAGLLSSVPDSAYEAVGATIGDA
AAAFAAELVLKVNAPDDAELAQMQSGSVLLGMLNPFDNACIARMAARGITAFALEAAPRT
SRAQSLDVLSSQANIAGYKAVLVGAHHYPRFMPMLMTAAGTVKAARVLILGAGVAGLQAI
ATAKRLGAVVEASDVRPAVKEQIESLGAKFVDVPLETDEERECAEGVGGYARPMPASWMA
RQAQAVHERALQSDIVITTALIPGRKAPTLLQEATVEQMKPGSVIVDLAAGHGGNCPLTE
IDQVVVRHGVTIVGHANLATLVPADASALYARNLLDFLKLVTDKDGQFQLNLEDDIVAAC
LMCRDGQVVRTNG