Protein Info for Psest_0414 in Pseudomonas stutzeri RCH2

Annotation: biotin biosynthesis protein BioC

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 264 PF01209: Ubie_methyltran" amino acids 11 to 161 (151 residues), 46.6 bits, see alignment E=9.7e-16 TIGR02072: malonyl-acyl carrier protein O-methyltransferase BioC" amino acids 16 to 262 (247 residues), 254.6 bits, see alignment E=5.7e-80 PF13489: Methyltransf_23" amino acids 37 to 192 (156 residues), 48.9 bits, see alignment E=2.3e-16 PF13847: Methyltransf_31" amino acids 51 to 160 (110 residues), 56.9 bits, see alignment E=6.7e-19 PF08242: Methyltransf_12" amino acids 52 to 143 (92 residues), 51.6 bits, see alignment E=4.6e-17 PF13649: Methyltransf_25" amino acids 53 to 141 (89 residues), 70.7 bits, see alignment E=5e-23 PF08241: Methyltransf_11" amino acids 53 to 145 (93 residues), 83.8 bits, see alignment E=3.9e-27 PF05148: Methyltransf_8" amino acids 96 to 146 (51 residues), 27.2 bits, see alignment 1.3e-09

Best Hits

Swiss-Prot: 72% identical to BIOC_PSEA7: Malonyl-[acyl-carrier protein] O-methyltransferase (bioC) from Pseudomonas aeruginosa (strain PA7)

KEGG orthology group: K02169, biotin synthesis protein BioC (inferred from 84% identity to psa:PST_3863)

Predicted SEED Role

"Biotin synthesis protein BioC"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GI11 at UniProt or InterPro

Protein Sequence (264 amino acids)

>Psest_0414 biotin biosynthesis protein BioC (Pseudomonas stutzeri RCH2)
MTDAALLPDKRQVAASFSRAAATYDSVAALQRQVGNQLLARLPAGAQPLSWIDLGSGTGF
FSRALAANFPRAEGIALDIAEGMLRHARPQGGALHFVAGDAERLPLRDGSVDLIYSSLAL
QWCEDFASVLREARRVLRSGGTLAFSSLCTGTLQELRDAWQAVDGFAHVNRFRSLATYQA
LCRDSGLAMVSLDVRPEVLHFPDLRQLTGELKALGAHNLNPGRPGGLTGRARIRALIDAY
EGFRQPAGLPATYQVVYGVLRKDS