Protein Info for Psest_0412 in Pseudomonas stutzeri RCH2

Annotation: 8-amino-7-oxononanoate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 91% identical to BIOF_PSEU5: 8-amino-7-oxononanoate synthase (bioF) from Pseudomonas stutzeri (strain A1501)

KEGG orthology group: K00652, 8-amino-7-oxononanoate synthase [EC: 2.3.1.47] (inferred from 91% identity to psa:PST_3865)

MetaCyc: 39% identical to BioF (Lysinibacillus sphaericus)
8-amino-7-oxononanoate synthase. [EC: 2.3.1.47]

Predicted SEED Role

"8-amino-7-oxononanoate synthase (EC 2.3.1.47)" in subsystem Biotin biosynthesis (EC 2.3.1.47)

MetaCyc Pathways

biotin biosynthesis I (13/15 steps found)
8-amino-7-oxononanoate biosynthesis I (9/11 steps found)
8-amino-7-oxononanoate biosynthesis IV (4/5 steps found)
biotin biosynthesis II (4/6 steps found)
8-amino-7-oxononanoate biosynthesis II (1/2 steps found)
8-amino-7-oxononanoate biosynthesis III (1/2 steps found)

KEGG Metabolic Maps

Biotin metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.3.1.47

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GE78 at UniProt or InterPro

Protein Sequence (392 amino acids)

>Psest_0412 8-amino-7-oxononanoate synthase (Pseudomonas stutzeri RCH2)
MTFDLASRLAERRAAHLYRQRPLLETPQQPEVMVDGERLLAFCSNDYLGLASHPEVIAAM
QQGAAKWGVGGGASHLVIGHSTPHHQLEEALADFTGRPRALLFSTGYMANLAAVTALVGQ
GDTVLEDRLNHASLLDAGLLSGARFSRYLHNDAEILAKRLDKAGGNTLVVTDGVFSMDGD
LADLPAICATAKPRGAWVMVDDAHGFGPLGATGGGIVEQFGLGIDDVPVLVGTLGKAFGT
AGAFVAGSEELIETLIQFARPYIYTTSQPPAVACATLKSLELLSSEGWRREHLNRLVARF
REGAQQIGLTLMDSPTPIQPVLVGSSERALRLSALLRERGILVGAIRPPTVPAGSARLRI
TFTASHSDAQLERLLEALAECWALLPKESDDA