Protein Info for Psest_4136 in Pseudomonas stutzeri RCH2

Annotation: TonB-dependent siderophore receptor

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 737 signal peptide" amino acids 1 to 29 (29 residues), see Phobius details PF07715: Plug" amino acids 89 to 185 (97 residues), 65 bits, see alignment E=8.5e-22 TIGR01783: TonB-dependent siderophore receptor" amino acids 91 to 737 (647 residues), 347.6 bits, see alignment E=8.4e-108 PF00593: TonB_dep_Rec_b-barrel" amino acids 266 to 702 (437 residues), 152.1 bits, see alignment E=4.6e-48

Best Hits

KEGG orthology group: K02014, iron complex outermembrane recepter protein (inferred from 74% identity to psa:PST_0147)

Predicted SEED Role

"Ferrichrome-iron receptor" in subsystem Iron acquisition in Vibrio or Transport of Iron

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GRH9 at UniProt or InterPro

Protein Sequence (737 amino acids)

>Psest_4136 TonB-dependent siderophore receptor (Pseudomonas stutzeri RCH2)
MSTLRFPRKSALLPVCLAASFAGSPLMAQERQPTQSAPASSQVSEPVELESVEITASADA
SAEGLTQPYAGEQVARGGRVGILGNRDYMETPFTSTSYTSQLIQDQQARSVSDVLQNDPS
VRVARGFGNFQELYVVRGFPVYSDDISYNGLYGLLPRQYVAAEFIERVEVFRGANTFLNG
AAPGGSGIGGAINILPKRAPNEPLTRLTVGAENGGQPMTHADIGRRFGEEERFGVRLNAA
KRAGETTVEDEDRTLDMFALGLDYQGDNFRLSSDIGHQYHFIDNPRPSVTPSGGIPSAPD
ADDNFAQPWTYSKEKQTFGTFRAEYDFSDAVTGWLAAGVREGEEKNRFASMTAGSSGAGT
FYRFDNVREEEVFTSEVGLRARGRTGDVSHEWVMSAAMFDLEARNAYAMSGSYAADIYQP
FAAAMPDAVWVGGSMSNPNVTERTQTQSLAIADTLGFMDDRLLVTLGARRQGIEAKSYDY
NSGDRLSSYNRYKNSPVAGVVYQLTDEVSVYANYIEGLVKGDIAPATSGGVSIINAGEAL
EPYVSEQTEIGIKYDGGSLGGSLALFTTERPFSTLENGVFTDGGEQRNRGIELSVFGEPT
YGVRLLGGVTLLDAELTSTQDGINEGNRAIGVPRTQANVGGEWDVPGTNGLTLTSRVVYT
SSQYTDLDNSREIPSWTRLDLGARYGLRVQDRDVTLRARLDNATGRDYWASTGGYANENY
LVLGAPRTLSVSATVDF