Protein Info for HP15_3996 in Marinobacter adhaerens HP15

Annotation: F0F1 ATP synthase subunit alpha

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 505 TIGR00962: ATP synthase F1, alpha subunit" amino acids 21 to 485 (465 residues), 593 bits, see alignment E=2.4e-182 PF02874: ATP-synt_ab_N" amino acids 23 to 87 (65 residues), 38.3 bits, see alignment E=2.4e-13 PF00006: ATP-synt_ab" amino acids 145 to 360 (216 residues), 271.4 bits, see alignment E=8.2e-85 PF00306: ATP-synt_ab_C" amino acids 367 to 490 (124 residues), 101.8 bits, see alignment E=5.3e-33

Best Hits

Swiss-Prot: 70% identical to ATPA2_NITEC: ATP synthase subunit alpha 2 (atpA2) from Nitrosomonas eutropha (strain DSM 101675 / C91)

KEGG orthology group: K02111, F-type H+-transporting ATPase subunit alpha [EC: 3.6.3.14] (inferred from 70% identity to net:Neut_2020)

MetaCyc: 49% identical to ATP synthase F1, alpha subunit (Synechococcus elongatus PCC 7942 = FACHB-805)

Predicted SEED Role

"ATP synthase alpha chain (EC 3.6.3.14)" in subsystem F0F1-type ATP synthase (EC 3.6.3.14)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.14

Use Curated BLAST to search for 3.6.3.14

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See E4PKT8 at UniProt or InterPro

Protein Sequence (505 amino acids)

>HP15_3996 F0F1 ATP synthase subunit alpha (Marinobacter adhaerens HP15)
MTPAEDFLDALLDVPAPSPALTEVGTVAEVGDGIAVVAGLARALADELLVFASGVRGVVL
DLEPDRLGVILLGPSDRITAGEDVWRTHKVFSVPVGNGLLGRILDATGGPRDGNGSVAST
AERPVEASAPGILSRAPVNRPLATGIKVIDGAVPIGLGQRELIIGDRQTGKTSIAIDTIL
NQARSDVVSIYCAIGQRGDAVARVIETLRKSGQLANTVVVAAGDEDAPGLAYVAPYAAMT
MAEYFADQGRDVLVVFDDLTHHARSYRELSLLLRRPPGREAFPGDIFYVHARLLERAGQF
TPEAGGGSITALPVVETQAENLSAYIPTNLISITDGQIYLSPRLVRKNQFPAVDIGLSVS
RVGGKAQHRAFREVAGNLRVTLSQFEELEDFARFGTRLDDNTRARLTRGAAVRTALRQPE
RDPMPAVEQLLVLMAALEGLLDGLSENEVAETMIRMRNSVGDSLKDVDESIRQNRSLEEA
EKQSLLRVARAALAVSGEDRHGPDA