Protein Info for Psest_4105 in Pseudomonas stutzeri RCH2

Annotation: formate dehydrogenase, beta subunit, Fe-S containing

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01582: formate dehydrogenase, beta subunit
  • amino acids 5 to 286 (282 residues), 458.3 bits, E=4.2e-142
• PF12797: Fer4_2
  • amino acids 28 to 45 (18 residues), 24.7 bits, see alignment (E = 7.9e-09)
• PF12800: Fer4_4
  • amino acids 32 to 46 (15 residues), 16.5 bits, see alignment (E = 4.5e-06)
  • amino acids 128 to 142 (15 residues), 14.9 bits, see alignment (E = 1.4e-05)
• PF12838: Fer4_7
  • amino acids 33 to 111 (79 residues), 35.7 bits, E=4.9e-12
• PF13247: Fer4_11
  • amino acids 89 to 185 (97 residues), 86.5 bits, E=5.9e-28
• PF13237: Fer4_10
  • amino acids 91 to 141 (51 residues), 27.7 bits, 1e-09
• PF00037: Fer4
  • amino acids 124 to 144 (21 residues), 25.4 bits, see alignment (E = 4.8e-09)
• PF09163: Form-deh_trans
  • amino acids 243 to 285 (43 residues), 80 bits, 4.4e-26

Best Hits

Swiss-Prot: 67% identical to FDOH_SHIFL: Formate dehydrogenase-O iron-sulfur subunit (fdoH) from Shigella flexneri

KEGG orthology group: K00124, formate dehydrogenase, beta subunit [EC: 1.2.1.2] (inferred from 97% identity to psa:PST_0165)

MetaCyc: 67% identical to formate dehydrogenase O subunit beta (Escherichia coli K-12 substr. MG1655)
FORMATEDEHYDROG-RXN [EC: 1.17.5.3]

Predicted SEED Role

"Formate dehydrogenase O beta subunit (EC 1.2.1.2)" in subsystem Formate dehydrogenase or Formate hydrogenase (EC 1.2.1.2)

MetaCyc Pathways

• nitrate reduction III (dissimilatory) (2/2 steps found)
• formate oxidation to CO₂ (1/1 steps found)
• formate to dimethyl sulfoxide electron transfer (1/2 steps found)
• formate to trimethylamine N-oxide electron transfer (1/2 steps found)
• formate to nitrite electron transfer (1/3 steps found)
• purine nucleobases degradation II (anaerobic) (16/24 steps found)
• oxalate degradation VI (1/4 steps found)
• oxalate degradation III (1/5 steps found)
• superpathway of C1 compounds oxidation to CO₂ (4/12 steps found)
• purine nucleobases degradation I (anaerobic) (6/15 steps found)

KEGG Metabolic Maps

• Glyoxylate and dicarboxylate metabolism
• Methane metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.2.1.2

Use Curated BLAST to search for 1.17.5.3 or 1.2.1.2

Sequence Analysis Tools

See L0GT72 at UniProt or InterPro

Protein Sequence (312 amino acids)

>Psest_4105 formate dehydrogenase, beta subunit, Fe-S containing (Pseudomonas stutzeri RCH2)
MATQDVIARSATTTERPSIREIGEVAKLIDTSKCIGCKACQVACSEWNDLRDDVGTSNGT
YDNPIDLTAESWTVMKFAEYENPGSGNLEWLIRKDGCMHCADPGCLKACPAPGAIVKYAN
GIVDFNQDHCIGCGYCITGCPFDIPRISEKDKKAYKCTLCSDRVSVGLEPACVKTCPTGA
IVFGSKEDMKEHAAGRIADLKERGFDQAGLYDPDGVGGTHVMYVLHHADQPSLYSDLPNE
PTISPLVSLWKGVTKPLALLGMGAAVLAGFFHYTRVGPIRVEEDENKTELSEPVVHQVDP
SVHVVDPKEPRP