Protein Info for Psest_4075 in Pseudomonas stutzeri RCH2

Updated annotation (from data): symporter for L-glutamate, L-glutamine, and L-proline
Rationale: Specifically important for utilization of L-glutamate, L-glutamine, or L-proline as carbon soruces. Glutamine is probably cleaved by a cytoplasmic glutaminaes (Psest_4081), so glutamine is probably a substrate. Mildly detrimental during tyrosine utilization, so tyrosine might also be a substrate.
Original annotation: Na+/H+-dicarboxylate symporters

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 419 transmembrane" amino acids 19 to 39 (21 residues), see Phobius details amino acids 48 to 71 (24 residues), see Phobius details amino acids 88 to 109 (22 residues), see Phobius details amino acids 152 to 170 (19 residues), see Phobius details amino acids 193 to 212 (20 residues), see Phobius details amino acids 220 to 247 (28 residues), see Phobius details amino acids 255 to 276 (22 residues), see Phobius details amino acids 296 to 321 (26 residues), see Phobius details amino acids 333 to 350 (18 residues), see Phobius details amino acids 356 to 377 (22 residues), see Phobius details PF00375: SDF" amino acids 19 to 403 (385 residues), 446.5 bits, see alignment E=4.3e-138

Best Hits

Swiss-Prot: 35% identical to DCTA_AGRVS: C4-dicarboxylate transport protein (dctA) from Agrobacterium vitis (strain S4 / ATCC BAA-846)

KEGG orthology group: None (inferred from 98% identity to psa:PST_0196)

MetaCyc: 31% identical to C4 dicarboxylate/orotate:H+ symporter (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-121; TRANS-RXN-121A; TRANS-RXN-121C; TRANS-RXN-122A; TRANS-RXN0-451; TRANS-RXN0-517; TRANS-RXN0-553

Predicted SEED Role

No annotation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GT47 at UniProt or InterPro

Protein Sequence (419 amino acids)

>Psest_4075 symporter for L-glutamate, L-glutamine, and L-proline (Pseudomonas stutzeri RCH2)
MTQSTTLSAPLRLLVRLPLWQQILIGLALGVAAGMAFGADAQLLAPIGTLFLNAIKMLIV
PLVFVSLVAGITSMQDSAKLGRISLKTIAIYLVTTAFAVSIGLLFGALFSPGEGMNMVAS
GNEQAKQAPSLVSILVGLVPANPVTAFAEGNILQIIVFAIALGVSINLIGERGAPAVRLF
DALAETFYKLTDLVMRVAPIGVFALTAGVVGSHGAEVLLPLAGVIGVIYLASIAHVLLVY
GGLLGLLARLNPLRFFQGIAPALAVAFSTSSSSGTLPVSIECARKNLGVSEGVAGFVLPV
GATINMDGTAIYQGVLALFIAQAFGIDLSAGQYAMIILTATLASIGTAGIPGAGLIMLGL
VLTAAGLPLEGVALIAGIDRILDMARTTVNVAGDLMTTTLVGRSEQELDRAIYDSSNKE