Protein Info for Psest_4044 in Pseudomonas stutzeri RCH2

Annotation: type I site-specific deoxyribonuclease, HsdR family

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1049 PF04313: HSDR_N" amino acids 1 to 208 (208 residues), 162.9 bits, see alignment E=2.3e-51 TIGR00348: type I site-specific deoxyribonuclease, HsdR family" amino acids 4 to 723 (720 residues), 614.4 bits, see alignment E=1.4e-188 PF18766: SWI2_SNF2" amino acids 267 to 532 (266 residues), 255.3 bits, see alignment E=1.3e-79 PF04851: ResIII" amino acids 307 to 469 (163 residues), 70.2 bits, see alignment E=5.2e-23 PF22679: T1R_D3-like" amino acids 631 to 705 (75 residues), 76.5 bits, see alignment 8.2e-25 PF11867: T1RH-like_C" amino acids 723 to 1045 (323 residues), 425.4 bits, see alignment E=4.4e-131

Best Hits

KEGG orthology group: K01153, type I restriction enzyme, R subunit [EC: 3.1.21.3] (inferred from 69% identity to maq:Maqu_3386)

Predicted SEED Role

"Type I restriction-modification system, restriction subunit R (EC 3.1.21.3)" in subsystem Restriction-Modification System (EC 3.1.21.3)

Isozymes

Compare fitness of predicted isozymes for: 3.1.21.3

Use Curated BLAST to search for 3.1.21.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GTA1 at UniProt or InterPro

Protein Sequence (1049 amino acids)

>Psest_4044 type I site-specific deoxyribonuclease, HsdR family (Pseudomonas stutzeri RCH2)
MLNEQQLEELCLNWFQQVGWRFAHGPDIAPDSSAPERTDYRQVVLRERLLAALARINPQI
PAAALEQAVHALQTVSEPQMVVRNRSVHRLLLSGVAVQFEIGEQKKQDWVYFIDFAQPSN
NDFLVVNQFTITGTRQPRRPDLIAFVNGLPLAVVELKNPANEQTDVWDAFNQLQTYKDEI
SDLFNSNAALVVSDGWTARVGSLTANAERMLPWRTVLNEDDRPRLQMELETVVRGFFKPE
LFLDYVRHFVLFEQDGDSIVKKIAGYHQFHAVREAVRATVIASQHADKGVLAIHEERATY
AKEVKPGSRKAGVVWHTQGSGKSITMACYAGKLLQQSEMKNPTLVVVTDRNDLDGQLFAT
FCAASDLLKTTPQQAGDRDELREMLASREAGGIIFTTVQKFALQEDEEKHPLLSDRTNIV
VISDEAHRSQYGSKGRLDTKTGKYVFGYAKHLHDALKNATFLGFTGTPIALEDRDTREVF
GDYVSIYDIQDAVDDGATVPIFYESRLAKLDVNQAEIDTLNEQVEEVVEDEEDIVSREKT
KGEWAALEKLVGSEPRLQQVARDLIEHFEARVSVADGKAMIVCMSRDICAHLYNAIVSLR
PDWHSDDPEQGAIKIVMTGSAADKALLQPHLYSKQVKKRLETRFKDVKDPLKLVIVRDMW
LTGFDAPCCHTMYIDKPMKGHNLMQAIARVNRVFKNKPGGLVVDYIGIANELKAALKTYT
DAKGKGDPTHNSAEALAVLLEKMDIVRGMMHGFDYSAFETQPLELLVPVSNHILGLKDGK
TRFLDTMLAVTKAFSLCGTLDEAAPLRKEIAFFSAIKAAITKFTTVDKKRSDAEKNSALK
QILDNAITSDGVDDIFALAGLDKPNIGLLSDEFLEDVRQMESRNLAVELLEKLLRDEVKA
RARNNVVQEKKYGDRLLETLRRYHNRAIETAQVIEELIQMAKEFKAALEREAALGLSHDE
IAFYDALANNESAVRELGDETLKKIAAELTEKLRASTTIDWQVRESVRAKLRILVRRCLQ
RWKYPPDQQSDAVELVLKQAESLSNEWSL