Protein Info for HP15_3909 in Marinobacter adhaerens HP15

Annotation: carboxylesterase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02821: S-formylglutathione hydrolase
  • amino acids 2 to 278 (277 residues), 420.4 bits, E=1.8e-130
• PF00756: Esterase
  • amino acids 21 to 272 (252 residues), 204.6 bits, E=1.1e-64

Best Hits

Swiss-Prot: 56% identical to ESTD_RAT: S-formylglutathione hydrolase (Esd) from Rattus norvegicus

KEGG orthology group: K01070, S-formylglutathione hydrolase [EC: 3.1.2.12] (inferred from 86% identity to maq:Maqu_0257)

MetaCyc: 56% identical to S-formylglutathione hydrolase / S-lactoylglutathione hydrolase (Escherichia coli K-12 substr. MG1655)
Hydroxyacylglutathione hydrolase. [EC: 3.1.2.6]; S-formylglutathione hydrolase. [EC: 3.1.2.6, 3.1.2.12]

Predicted SEED Role

"S-formylglutathione hydrolase (EC 3.1.2.12)" in subsystem Glutathione-dependent pathway of formaldehyde detoxification (EC 3.1.2.12)

MetaCyc Pathways

• formaldehyde oxidation II (glutathione-dependent) (3/3 steps found)
• methylglyoxal degradation I (2/3 steps found)
• methylglyoxal degradation VIII (2/3 steps found)
• superpathway of methylglyoxal degradation (4/8 steps found)
• superpathway of C1 compounds oxidation to CO₂ (4/12 steps found)

KEGG Metabolic Maps

• Methane metabolism
• Pyruvate metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.1.2.6

Use Curated BLAST to search for 3.1.2.12 or 3.1.2.6

Sequence Analysis Tools

See E4PJW3 at UniProt or InterPro

Protein Sequence (280 amino acids)

>HP15_3909 carboxylesterase (Marinobacter adhaerens HP15)
MELLSTNVSFEGEHRRYRHTSATLECDMEFAVYLPPVAVGSNPKKVPVLYWLSGLTCTDQ
NFMQKAGAQKRAAQLGMAIVCPDTSPRGVNLPGEDDSYDFGSGAGFYINATEQPWAPHYR
MYDYVVKELPQLIENALPVTDKRSVSGHSMGGHGALICALKNPGRYASVSAFAPIANPTE
CPWGQKAFKGYLGDDPRSWEEWDATLLIPTARERLPLLVDQGTADEFLDSQLNPDALADA
CEKFHHHINLRMHRGYDHSYFFIASFIDDHLDHHAKALGL