Protein Info for Psest_0397 in Pseudomonas stutzeri RCH2

Annotation: Small-conductance mechanosensitive channel

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1096 signal peptide" amino acids 1 to 22 (22 residues), see Phobius details transmembrane" amino acids 480 to 496 (17 residues), see Phobius details amino acids 524 to 547 (24 residues), see Phobius details amino acids 553 to 575 (23 residues), see Phobius details amino acids 603 to 620 (18 residues), see Phobius details amino acids 631 to 648 (18 residues), see Phobius details amino acids 668 to 689 (22 residues), see Phobius details amino acids 702 to 723 (22 residues), see Phobius details amino acids 771 to 793 (23 residues), see Phobius details amino acids 813 to 833 (21 residues), see Phobius details amino acids 854 to 876 (23 residues), see Phobius details amino acids 887 to 914 (28 residues), see Phobius details PF12795: MscS_porin" amino acids 35 to 259 (225 residues), 191.6 bits, see alignment E=4.5e-60 PF12794: MscS_TM" amino acids 480 to 799 (320 residues), 350.7 bits, see alignment E=1.9e-108 PF21088: MS_channel_1st" amino acids 860 to 901 (42 residues), 30.7 bits, see alignment (E = 6.3e-11) PF00924: MS_channel_2nd" amino acids 903 to 968 (66 residues), 83.2 bits, see alignment 2.8e-27 PF21082: MS_channel_3rd" amino acids 976 to 1059 (84 residues), 65.6 bits, see alignment 1.2e-21

Best Hits

KEGG orthology group: K05802, potassium efflux system protein KefA (inferred from 94% identity to psa:PST_3880)

Predicted SEED Role

No annotation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GE62 at UniProt or InterPro

Protein Sequence (1096 amino acids)

>Psest_0397 Small-conductance mechanosensitive channel (Pseudomonas stutzeri RCH2)
MSLLRTLLFSTICLFVAPLHAQSLDSLTGTPPATEGEAKPAQLSLGEQTQRMVQQTETSN
KRAEDLKALLAQAPKEIAEAQRELAKLKASPDEDPAQRYAKQSVEALEQRLSARVEELSE
WQKQFSAANSMIITAQTRPERAQAQISTAQTRIQEINNLLKSGRESGKPLSEERRGLLDA
EIVSLSAQIDLRRQELAGNSLLQDLGKARRDLLAERIARAEQDTQALQSLINEKRRAESE
QTVAEFSARVQQAGSDKLLAAESAENLKLSDYLLRATERLNRLNQQNLRTRQQLDTLNQT
DQALEEQIAVLEGSLLLSRILYQQKQALPSLQLDKNLADEIADIRLYQFELNQRREAAGN
PAAYVEQLLAQQKEEEITPELRRTLMDLATTRSELLDRLNRELDALLNASITLQLNQKQL
QDTARTLSDTLDEQMFWIPSNKPLDLGWLQGSVQRLKAQLAAMPWLSAIQELGAGLKERP
LFFLPLLLLIAGLLWWRKTIDAKLSSLSEQIGHFRNDSQLHTPLALLLNLLLALPGALFL
ALCGYLLQMDARGQNYVLGAALYEMAQAWLVFYSAYRMLSPGRVAELHFGWSRPQVAFLR
DEIRRLGMIVMALVAVVSVAEHQPARLADDVLGILVVLACFALMSWRLNRLLLKGPSSQN
APPLRLMIGLLFSMLPIALIVAVGFGYYYTALKLTDRLIDTLYLLMIWIVVEAALIRGLT
VAARRLAYKRALAKRQAQTEEPGDPVETQGEPGLDIEQVNQQSLRLTRMTMFGVFLVALY
WVWSDLISVVSYLDNITLYEYTSGSGDALTTAAISLNNLLGALLIIAITVALARNLPGLL
EVMVLSKLRLAQGSAYATTTLLSYALAGFGIVATLSTLGVSWDKLQWLVAALSVGLGFGL
QEIFANFISGLIILFERPVRIGDVVTIGNLSGTVSRIRIRATTITDFDRKDIIVPNKTFI
TGQLINWSLSDTVTRVTLKVGVAYGSDLEQVKTLLYKAAQANPRVLKDPEPQVFFLNFGE
STLDHELRIHVRDLGDRNPATDEINRFIDREFNKAGINIAFRQVDVFLKNFAGQQLQLSA
TPKPADEQKPSTDDRG