Protein Info for Psest_3936 in Pseudomonas stutzeri RCH2

Annotation: ATP-dependent protease HslVU, peptidase subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 178 TIGR03692: ATP-dependent protease HslVU, peptidase subunit" amino acids 2 to 174 (173 residues), 275.2 bits, see alignment E=9.5e-87 PF00227: Proteasome" amino acids 2 to 172 (171 residues), 79.7 bits, see alignment E=1.1e-26

Best Hits

Swiss-Prot: 93% identical to HSLV_PSEAE: ATP-dependent protease subunit HslV (hslV) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K01419, ATP-dependent HslUV protease, peptidase subunit HslV [EC: 3.4.25.2] (inferred from 100% identity to psa:PST_0345)

MetaCyc: 75% identical to peptidase component of the HslVU protease (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"ATP-dependent protease HslV (EC 3.4.25.-)" in subsystem Proteasome bacterial or Proteolysis in bacteria, ATP-dependent (EC 3.4.25.-)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.4.25.- or 3.4.25.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GSQ2 at UniProt or InterPro

Protein Sequence (178 amino acids)

>Psest_3936 ATP-dependent protease HslVU, peptidase subunit (Pseudomonas stutzeri RCH2)
MTTIVSVRRNGKVVMGGDGQVSLGNTVMKGNAKKVRRLYHGQVLAGFAGATADAFTLFER
FEGQLEKHQGHLVRAAVELAKDWRTDRSLSRLEAMLAVANKDASLIITGNGDVVQPEDDL
IAMGSGGGFAQAAAKALLLKAGADMSAQEIAETALNIAGSICVFTNQNLTIEELDSAV