Protein Info for Psest_3905 in Pseudomonas stutzeri RCH2

Annotation: formate dehydrogenase, alpha subunit, archaeal-type

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 698 PF04879: Molybdop_Fe4S4" amino acids 1 to 47 (47 residues), 62.7 bits, see alignment 3.9e-21 TIGR01591: formate dehydrogenase, alpha subunit" amino acids 7 to 673 (667 residues), 765.3 bits, see alignment E=2.3e-234 PF00384: Molybdopterin" amino acids 58 to 480 (423 residues), 271.4 bits, see alignment E=1.7e-84 PF01568: Molydop_binding" amino acids 566 to 671 (106 residues), 95.1 bits, see alignment E=4.1e-31

Best Hits

KEGG orthology group: K00123, formate dehydrogenase, alpha subunit [EC: 1.2.1.2] (inferred from 47% identity to rci:RCIX2378)

Predicted SEED Role

"Assimilatory nitrate reductase large subunit (EC:1.7.99.4)" in subsystem Nitrate and nitrite ammonification (EC 1.7.99.4)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.2.1.2, 1.7.99.4

Use Curated BLAST to search for 1.2.1.2 or 1.7.99.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GSM0 at UniProt or InterPro

Protein Sequence (698 amino acids)

>Psest_3905 formate dehydrogenase, alpha subunit, archaeal-type (Pseudomonas stutzeri RCH2)
MQVVPSICTFCGVGCGLGLRVEGGRVVGVEPQPGHPVSAGQLCVKGWASSFAVDVGNRLR
TPLIKEHGRFREASWDEALALVASEFRAALDTAGPSSVGVISCAHATNEDNYAAQKFARA
VLGTANIDHCARICHSPSVAGLGRSLGSGAMTNSIADIDEAELILVIGADVTENHAMIGA
RMLRAQARGARLIVVDPRRTRLARLADQHVQLRVGSNIALLNGLLHIIFANAWEDRDFLA
CRCDNEAALRAHVRELTPELTSAQTGVPVLALIELARAYSQARRAFIAYGMGITQFQSGT
HNVIAVANLALVCGQLGRPGTGVNPLRGQNNVQGACDMGCLPDVYPGYQKVADAQVRAKF
ARAWGVAQPPAPGLTSLGMSEAALRGEFRALLVLGEELVVTDPDQHKLARALAALDFLVV
VELTLSETARFADVVLPAASFAEKDGTFSNCERRMQLLHKAVEPPGQARADWQILAALAE
CMGYDGMRWASAEEVFAEMAALAPPFSAISHARLEQGGGLQWPCDAAHPAGTAILHRQCF
PRGRAQLIPVSQVEPDECPDADYPFCFTTQRLHFHYGGGAMTRKSPLLERETPAGLLFIH
PQDAARLGLAEAQGVRVASRRGALETRAHLTDDVPPGTVAMPYHFREAPCNRLTNSAQDP
ISHMPELKACAVALTPLAPGQAPHSELERPQEAEHAGP