Protein Info for Psest_3888 in Pseudomonas stutzeri RCH2

Annotation: Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 474 transmembrane" amino acids 6 to 22 (17 residues), see Phobius details amino acids 29 to 49 (21 residues), see Phobius details amino acids 71 to 93 (23 residues), see Phobius details amino acids 105 to 122 (18 residues), see Phobius details amino acids 128 to 147 (20 residues), see Phobius details amino acids 156 to 177 (22 residues), see Phobius details amino acids 197 to 219 (23 residues), see Phobius details amino acids 230 to 252 (23 residues), see Phobius details amino acids 264 to 284 (21 residues), see Phobius details amino acids 292 to 314 (23 residues), see Phobius details amino acids 321 to 342 (22 residues), see Phobius details amino acids 363 to 383 (21 residues), see Phobius details amino acids 403 to 425 (23 residues), see Phobius details amino acids 446 to 469 (24 residues), see Phobius details PF00361: Proton_antipo_M" amino acids 122 to 411 (290 residues), 157.1 bits, see alignment E=3e-50

Best Hits

KEGG orthology group: K05903, NADH dehydrogenase (quinone) [EC: 1.6.99.5] (inferred from 95% identity to psa:PST_0383)

Predicted SEED Role

"Hydrogenase-4 component B / Formate hydrogenlyase subunit 3" in subsystem Formate hydrogenase

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.6.99.5

Use Curated BLAST to search for 1.6.99.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GNM6 at UniProt or InterPro

Protein Sequence (474 amino acids)

>Psest_3888 Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit (Pseudomonas stutzeri RCH2)
MNAALVSLLLPLAGALLLILLRPARSGRWVIALSLGALVAAIMALQVVLDHGERSLLVGG
WEAGLAIRFRLTPLAALLLVFTAGLHLLVALYAARIPHAAGKEDYWPLSCLLHAALAALW
LSADLFNLYVTLELLSLVAVALVSLAGRKAWKPALNYLLLSLAGSLAYLLGVALIYGRYG
VLDLAMLAQLSEADGVTHLALLLMTLGLMLKAALWPLHLWLPPAHAAAPTAVSALLSALV
VKGPLYILWLIWSEIAPAEFGRDAGLMFGAAGVLALLAGGWSALRAPRLKTLVAYSTVAQ
LGYALLALGLLLHLNEPRLHAALWLFVLAHGLAKVSMFLAAGELQSILGSKRVTGMRGAS
QNVPIALAAFAVAGGSLVGLPPSGGFLAKWLLLQPLFEQPQHWPWALGVLLGTLMSAAYV
FRVVAHGFDRARPNPPSIHPDRLAQWLALLPALLVWGLALISEPLLLWLGGLGR