Protein Info for HP15_3739 in Marinobacter adhaerens HP15

Annotation: glycyl-tRNA synthetase beta subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 693 TIGR00211: glycine--tRNA ligase, beta subunit" amino acids 1 to 689 (689 residues), 845.1 bits, see alignment E=3.1e-258 PF02092: tRNA_synt_2f" amino acids 6 to 550 (545 residues), 697.2 bits, see alignment E=1.8e-213 PF05746: DALR_1" amino acids 582 to 679 (98 residues), 43.4 bits, see alignment E=3.4e-15

Best Hits

Swiss-Prot: 90% identical to SYGB_MARHV: Glycine--tRNA ligase beta subunit (glyS) from Marinobacter hydrocarbonoclasticus (strain ATCC 700491 / DSM 11845 / VT8)

KEGG orthology group: K01879, glycyl-tRNA synthetase beta chain [EC: 6.1.1.14] (inferred from 90% identity to maq:Maqu_0038)

MetaCyc: 54% identical to glycine--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Glycine--tRNA ligase. [EC: 6.1.1.14]

Predicted SEED Role

"Glycyl-tRNA synthetase beta chain (EC 6.1.1.14)" (EC 6.1.1.14)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.1.1.14

Use Curated BLAST to search for 6.1.1.14

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See E4PI17 at UniProt or InterPro

Protein Sequence (693 amino acids)

>HP15_3739 glycyl-tRNA synthetase beta subunit (Marinobacter adhaerens HP15)
MATQDFLVELGTEELPPKALKPLSDAFTQGIARGLEEAGIEFGKVEAFAAPRRLAVRIRD
LADSQPDKSVEKRGPAVKAAFDDAGNPTRALTGFATSLGVTPDQLDTLETDKGAWVVYRT
VEQGKPTVELMPELVEQSLAGLPIPKRMRWGAHRTEFVRPVHWVVMLFGNKVIEAPIMGL
TPGNKTRGHRFHCPKSLIVPTPSDYEVVLKQEGYVIADFAERREQIRAGVTELAEKEAGG
KAVIDEDLLDEVTALNEWPVPLMGRFEERFLEVPAEALISSMKEHQKYFHVVAADGEMLP
LFITVANIESKDPSQVVSGNEKVIRPRLSDAAFFYETDRKTKLEDRIDALKPIVFQEKLG
SIYDKSVRVAALAKKIADAIGSDPALAERAAMLAKTDLVTEMVLEFTDLQGIMGQYYAAN
DGEPEDVAKALNEQYMPRFAGDDLPTTLTGCAIAIADRLDSLVGLFGINQPPSGTRDPFA
LRRASLGVLRIIIERELPLDLQTCCEWAEENFSVLTEQNTASTVVDYMLERFRAHYDEQG
IGAEVYLAVHARRPTRPLDFDRRVKAVEAFRQLPEAQALAGANKRVSNILTKQGGDSIGE
TVDASLLQDSAEKALAEQVDQQAAKVLPLFENGDYASALSSLASLRAPVDNFFDEVMVMA
DDEAVRNNRLALLNRLRNLFLRVADISLLPTAG