Protein Info for Psest_0380 in Pseudomonas stutzeri RCH2

Annotation: TonB-dependent siderophore receptor

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 707 signal peptide" amino acids 1 to 31 (31 residues), see Phobius details PF07715: Plug" amino acids 72 to 171 (100 residues), 78.3 bits, see alignment E=6.1e-26 TIGR01783: TonB-dependent siderophore receptor" amino acids 74 to 707 (634 residues), 359.3 bits, see alignment E=2.3e-111 PF00593: TonB_dep_Rec_b-barrel" amino acids 245 to 676 (432 residues), 218.1 bits, see alignment E=4.4e-68

Best Hits

KEGG orthology group: K02014, iron complex outermembrane recepter protein (inferred from 58% identity to azo:azo2997)

Predicted SEED Role

"Ferrichrome-iron receptor" in subsystem Iron acquisition in Vibrio or Transport of Iron

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GG82 at UniProt or InterPro

Protein Sequence (707 amino acids)

>Psest_0380 TonB-dependent siderophore receptor (Pseudomonas stutzeri RCH2)
MSRVVASPPFSLRPLVLGLPMLVACFAVEAQEAVELEAQQITAPREVERGNGPVEGYVAK
RSVSATKTDTPIIETPQSISVITADRIRDQGSLTIQDSLRYVAGMRGEAYGFDSRGDFAL
IRGVAPTLFLDGLQQSVGFYTNSRTDPFTLERVEVIKGPSSMLYGQSSVGGLLNFVSKRP
QAEQKSELQLQYGSFDRKQIAFDSTGPLDDDGTLLYRVVAIQRDSQTQVDHTKDNRLLFM
PSLTWRPNEQFEWTLIANVQKDDGGTTSGFLPHRGTVLSAPYGEIGSERFVSEPGFDEYD
TEQKALTSQMAWRLDDTWTLRQNLRWQKSKVSYQSIYGWPPVLGADDRTVNRVYYVSKPE
VTIWSADHQAESRFDTGPLQHTALVGIDYRHAVTDSRTARGNATPLDLYDPVYGTFDPSG
ITLSDVPQQRVAQQGLYVQDQIRLDKWLATLGLRKDWADTRVEQGTRQKDDAVTGRVGLT
YLFDNGVAPYISYSESFTPIIGLDNVTQQSYKPLEGEQWELGVKYQPAGSNTLLTAAVFD
LREKNRQMPNPANPLSTIQAGEARVKGLELEGLVEMNSNWDLIGTYTYLDSEVVKGPVAQ
QGKRIASVPEHMASLWSQHRFSIAGIPGFSAGAGVRYVGASWDGTDSLKTPSTTLFDAML
GYAYQDWSLTLNATNLEDETYYTTCLSRGDCFTGNRRTLTATASYSF