Protein Info for Psest_3844 in Pseudomonas stutzeri RCH2

Annotation: 4-hydroxybenzoate polyprenyl transferase, proteobacterial

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 297 transmembrane" amino acids 29 to 49 (21 residues), see Phobius details amino acids 55 to 76 (22 residues), see Phobius details amino acids 101 to 119 (19 residues), see Phobius details amino acids 125 to 141 (17 residues), see Phobius details amino acids 146 to 164 (19 residues), see Phobius details amino acids 176 to 195 (20 residues), see Phobius details amino acids 216 to 237 (22 residues), see Phobius details amino acids 243 to 261 (19 residues), see Phobius details amino acids 273 to 294 (22 residues), see Phobius details TIGR01474: 4-hydroxybenzoate polyprenyl transferase" amino acids 18 to 293 (276 residues), 352.5 bits, see alignment E=9.2e-110 PF01040: UbiA" amino acids 34 to 279 (246 residues), 239 bits, see alignment E=2.5e-75

Best Hits

Swiss-Prot: 98% identical to UBIA_PSEU5: 4-hydroxybenzoate octaprenyltransferase (ubiA) from Pseudomonas stutzeri (strain A1501)

KEGG orthology group: K03179, 4-hydroxybenzoate octaprenyltransferase [EC: 2.5.1.-] (inferred from 98% identity to psa:PST_0428)

MetaCyc: 72% identical to 4-hydroxybenzoate-polyprenyltransferase (Pseudomonas putida)
4-hydroxybenzoate nonaprenyltransferase. [EC: 2.5.1.39]

Predicted SEED Role

"4-hydroxybenzoate polyprenyltransferase (EC 2.5.1.39)" (EC 2.5.1.39)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.5.1.-

Use Curated BLAST to search for 2.5.1.- or 2.5.1.39

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GSN0 at UniProt or InterPro

Protein Sequence (297 amino acids)

>Psest_3844 4-hydroxybenzoate polyprenyl transferase, proteobacterial (Pseudomonas stutzeri RCH2)
MYLKLLQSCNRLHPRAWDFIQLMRLDKPIGTYLLLWPTLWALWIAAEGVPSAKNLFIFIA
GVILMRAAGCVVNDFADRNFDGHVQRTQARPMAAGRVSSREAWTLFAVLVGLSFGLVLLT
NATTVYLSFGALALAACYPFMKRYTFYPQVVLGAAFSWGMPMAFTAETGSLPPEAWLLFI
ANLLWTVAYDTYYAMADRDDDLKIGIKSTAILFGEADRVIIVTLQGLALFCLLLAGVRFE
LGQWFHLGLVVAASCFAWEFWKTQSRKPQICFKAFLHNHWAGLAILAGVVLDYATKA