Protein Info for PGA1_c03870 in Phaeobacter inhibens DSM 17395

Annotation: crotonyl-CoA reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 426 TIGR01751: crotonyl-CoA carboxylase/reductase" amino acids 31 to 420 (390 residues), 614.1 bits, see alignment E=4.6e-189 PF08240: ADH_N" amino acids 64 to 178 (115 residues), 59 bits, see alignment E=5e-20 PF00107: ADH_zinc_N" amino acids 222 to 366 (145 residues), 58.3 bits, see alignment E=8.3e-20

Best Hits

Swiss-Prot: 84% identical to CCR_RHOS4: Crotonyl-CoA reductase (ccr) from Rhodobacter sphaeroides (strain ATCC 17023 / 2.4.1 / NCIB 8253 / DSM 158)

KEGG orthology group: K14446, crotonyl-CoA carboxylase/reductase (inferred from 91% identity to sil:SPO0370)

MetaCyc: 74% identical to crotonyl-CoA carboxylase/reductase monomer (Methylorubrum extorquens AM1)
RXN-18384 [EC: 1.3.1.85]; 1.3.1.85 [EC: 1.3.1.85]

Predicted SEED Role

"Crotonyl-CoA carboxylase/reductase, ethylmalonyl-CoA producing"

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.3.1.85

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See I7DLV0 at UniProt or InterPro

Protein Sequence (426 amino acids)

>PGA1_c03870 crotonyl-CoA reductase (Phaeobacter inhibens DSM 17395)
MALDTQNGALSYEAPEKELYEIGELPPMGYVPQKMYAWTIRKERHGNPDTAMVQEVVDVP
KLDSHDVLVLVMAAGVNYNGVWAALGTPISPFDGHGAPFHIAGSDASGIVWAVGDKVKRW
KVGDEVVIHCNQDDGDDEECNGGDPMYSPSQRIWGYETPDGSFAQFTNVQSQQLMPRPKH
LTWEESACYTLTLATAYRMLFGHEPHDLKPGQNVLVWGASGGLGSYAIQLINTAGANAIG
VISDESKRDFVMGLGAKGVINRKDFNCWGQLPTVNTPEYANWFKEARKFGKAIWDITGKG
NNVDMVFEHPGESTFPVSTFVCKKGGMVVICAGTTGYNLTFDVRYMWMHQKRLQGSHFAH
LKQAAAANKLMVERRLDPCMSEVFSWSDLPDAHMKMLRNEHKPGNMSVLVQAPRTGLRTL
EDVLAG