Protein Info for Psest_3817 in Pseudomonas stutzeri RCH2

Annotation: (p)ppGpp synthetase, RelA/SpoT family

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 702 PF13328: HD_4" amino acids 26 to 175 (150 residues), 171.6 bits, see alignment E=3.8e-54 TIGR00691: RelA/SpoT family protein" amino acids 26 to 698 (673 residues), 793.6 bits, see alignment E=7.8e-243 PF01966: HD" amino acids 45 to 144 (100 residues), 44 bits, see alignment E=8.8e-15 PF04607: RelA_SpoT" amino acids 235 to 344 (110 residues), 144.5 bits, see alignment E=5.5e-46 PF02824: TGS" amino acids 389 to 448 (60 residues), 72.5 bits, see alignment 7.9e-24 PF19296: RelA_AH" amino acids 474 to 543 (70 residues), 34.3 bits, see alignment E=5.9e-12 PF28438: RelA_RIS" amino acids 559 to 618 (60 residues), 73.6 bits, see alignment 2.9e-24 PF13291: ACT_4" amino acids 623 to 699 (77 residues), 52.8 bits, see alignment E=1.8e-17

Best Hits

Swiss-Prot: 54% identical to SPOT_VIBCH: Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (spoT) from Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)

KEGG orthology group: K01139, guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase [EC: 3.1.7.2] (inferred from 99% identity to psa:PST_0459)

Predicted SEED Role

"GTP pyrophosphokinase (EC 2.7.6.5), (p)ppGpp synthetase II / Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (EC 3.1.7.2)" in subsystem Stringent Response, (p)ppGpp metabolism (EC 2.7.6.5, EC 3.1.7.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.6.5

Use Curated BLAST to search for 2.7.6.5 or 3.1.7.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See L0GR69 at UniProt or InterPro

Protein Sequence (702 amino acids)

>Psest_3817 (p)ppGpp synthetase, RelA/SpoT family (Pseudomonas stutzeri RCH2)
MPAIDALADRLSTYLGPDQVNLVRRAYFYAEQAHDGQRRRSGEAYVTHPLAVANILADMH
MDHQSLMAAMLHDVIEDTGIPKEALVAQFGETVAELVDGVSKLTQMNFETKAEAQAENFQ
KMAMAMARDIRVILVKLADRLHNMRTLEVLAGEKRRRIAKETLEIYAPIANRLGMHTMRV
EFEDLGFKAMHPMRSERIRAAVRRARGNRNEIVEKIEQSLIHCLEREGLDGEVLGREKHL
YSIYKKMRGKRKAFHEIMDVYAFRIVVDKADTCYRVLGAVHSLYKPLPGRFKDYIAIPKA
NGYQSLHTTLFGMHGVPIEIQIRTREMEEMANNGIAAHWLYKSPEDEVPKGTHARARQWV
KGVLELQQRAGNSLEFIESVKIDLFPDEVYVFTPKGRIMEMPKGSTAVDFAYAVHTDVGN
TCIACRVNRRLAPLSQPLESGSTVEIVTAPGARPNPAWLNFVVTGKARTHIRHALKLQRR
SESINLGERLLSKALSGFETSLEKISPERIQQVLTEYHMEYVEDLLEDIGLGNRMAYVIA
RRLILRDDEQAPALEGPLAIRGTEGLVLNYAKCCTPIPGDPIVGHLSAGKGMVVHLETCR
NIADVRHNPDKCIQLSWSKDVTGEFNVELRVELEHQRGLIALLAGSVNAADGNIEKIGMD
ERDGRISVVQLVVSVHDRVHLARVIKKLRAIKGVTRITRVKA